What are the constituents used for isolation of bacterial plasmid DNA, RNA and plant DNA respectively?

#Question id: 2066

#I Life Science/ Life Sciences Group – I-V

Which of the following statements describes a characteristic feature of a carrier protein in a plasma membrane?

#Question id: 4565

#I Life Science/ Life Sciences Group – I-V

Darwinʹs mechanism of natural selection required long time spans in order to modify species. From whom did Darwin get the concept of Earthʹs ancient age?

#Question id: 27832

#Research Methodology

Which country launched the ‘Beidou’ satellite navigation system?

#Question id: 566

#I Life Science/ Life Sciences Group – I-V

The role of the metal ion (Mg2+) in catalysis by enolase is to

#Question id: 13076

#SCPH06 I Botany

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.