Which enzyme converts the PPi to ATP in Pyrosequencing?

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#Question id: 2347

#SCPH28 | Zoology

Diffusion of molecules across biological membranes that requires channels is called

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#Question id: 15231

#SCPH01 Biochemistry

Which of the following is true for Capillary electrophoresis (CE) resolution

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#Question id: 17280

#SCPH01 Biochemistry

During DNA isolation, phenol / chloroform extraction is performed to remove

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#Question id: 17207

#SCPH05 I Biotechnology

A novel protein from a deep sea archaebacterium was identified and sequenced. The sequence is expected to be widely divergent from known sequences. Which scoring matrix will produce the most appropriate alignment in a search for homologs in the NCBI database?

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#Question id: 13062

#SCPH05 I Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?