Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm? 

P. A larger E-value indicates higher sequence similarity 

Q. E-value < 10^-10 indicates sequence homology 

R. A higher BLAST score indicates higher sequence similarity 

S. E-value > 10^10 indicates sequence homology 

Adding small double-stranded molecules with one internal site for a restriction endonuclease,termed ______ to cDNA.

Blood sugar levels are measured in milligrams of glucose per decilitre of blood volume. If a person’s blood sugar level measured 128 mg/dL, what is this in grams per litre?

Which of the following statement is incorrect regarding Clustal?

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition isolate a mutation that constitutively expresses abnormally high levels of ßgalactosidase, which you designate lacc. Preliminary P1 transduction experiments indicate that lacc  is linked to the Tn5 insertion.  To map lacc  relative to lac1– you set up two reciprocal crosses. In the first cross you grow P1 on a strain that carries the Tn5 insertion and the lac1– mutation. You then use this lysate to infect a lacc mutant and select for Kanr. From 100 Kanr transductants examined, 20 are Lac–, 76 express ß-galactosidase constitutively and 4 show normal ß-galactosidase expression. In the second cross you grow P1 on a strain that carries the Tn5 insertion and the lacc mutation. You then use this lysate to infect a lac1– mutant, and select for Kanr. From 100 Kanr transductants examined, 81 are Lac– and 19  express ß-galactosidase constitutively. So what will be the correct order of  the Tn5 insertion and the lac1– and lacc  mutations. Express any measured distances as cotransduction frequencies.