TLS Online TPP Program

#Question id: 17274


which of the following statement is incorrect about PSI-BLAST?

#SCPH01 Biochemistry
  1. The main feature of PSI-BLAST is that profiles are constructed automatically and are fine-tuned in each successive cycle. 
  2. PSI-BLAST employs a weighting scheme in the profile construction in each iteration to increase sensitivity.
  3. To increase sensitivity of PSI-BLAST, it uses pseudocounts to provide extra weight to unobserved residues to make the profile more inclusive.
  4. None of the above.
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TLS Online TPP Program

#Question id: 15656

#SCPH06 I Botany

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. Explain the two regulatory pathways for Mel1 that can explain the behavior of the MelA– and MelB– mutations. For each regulatory pathways what is a role for the inducer melibiose?

TLS Online TPP Program

#Question id: 15657

#SCPH01 Biochemistry

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium.
You next construct a MelA– MelB– double mutant, which gives the following behavior:
 
Which of your two models is consistent with this new data?
a) the MelA- MelB- double mutant is identical to the MelB- mutant
b) MelA- is epistatic to MelB-
c) MelB- is epistatic to MelA-
d) the MelA- MelB- double mutant is identical to the MelA- mutant
Which of the following is the correct prediction about the double mutants?

TLS Online TPP Program

#Question id: 15657

#SCPH06 I Botany

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium.
You next construct a MelA– MelB– double mutant, which gives the following behavior:
 
Which of your two models is consistent with this new data?
a) the MelA- MelB- double mutant is identical to the MelB- mutant
b) MelA- is epistatic to MelB-
c) MelB- is epistatic to MelA-
d) the MelA- MelB- double mutant is identical to the MelA- mutant
Which of the following is the correct prediction about the double mutants?

TLS Online TPP Program

#Question id: 15658

#SCPH01 Biochemistry

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium.
Next, you isolate a third mutant, MelC–, which gives constitutive melibiase expression. The MelC– mutation is closely linked to Mel1 and MelB–. Genetic tests of the MelC– mutation yield the following:
 
As above, classify the MelC– mutation in terms of its basic genetic properties and explain how you arrived at your conclusions.

TLS Online TPP Program

#Question id: 15658

#SCPH06 I Botany

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium.
Next, you isolate a third mutant, MelC–, which gives constitutive melibiase expression. The MelC– mutation is closely linked to Mel1 and MelB–. Genetic tests of the MelC– mutation yield the following:
 
As above, classify the MelC– mutation in terms of its basic genetic properties and explain how you arrived at your conclusions.

TLS Online TPP Program

#Question id: 15661

#SCPH12 I Genetics

Which of the following is NOT true of a maternal effect gene?