Which methods can be used for detection of self complementary regions of a sequence.

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#Question id: 2645

#SCPH28 | Zoology

When uncharged tRNA concentration is low what will you expect as the activity of tryptophan operon?

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#Question id: 15617

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

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#Question id: 1308

#SCPH28 | Zoology

What are the cytoskeletal proteins associated with hemidesmosomes?

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#Question id: 16130

#SCPH06 I Botany

You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.              

How many units of ß-galactiosidase would you expect to be expressed from deletion 1 in a gln2– mutant? 

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#Question id: 1080

#SCPH06 I Botany

 In the formation of biofilms, such as those forming on unbrushed teeth, cell signaling serves which function?