TLS Online TPP Program

#Question id: 595


It is difficult to determine either Km or Vmax from a graph of velocity vs. substrate concentration because

#1. Molecules and their Interaction Relevant to Biology
  1. too much substrate is required to determine them.

  2. the graph is sigmoidal.

  3. an asymptotic value must be determined from the graph.

  4. the points on the graph are often not spread out on the hyperbola.