TLS Online TPP Program

#Question id: 5761


E. coli cells were spread on an agar plate, producing 1000 colonies.  The colonies are replica plated on two agar plates containing the antibiotic kanamycin and one agar plate without antibiotics.  All of the colonies are able to grow on the agar plate without antibiotic but only 4 colonies are able to grow on each of the agar plates containing kanamycin.  You notice that the four colonies that grew on each of the kanamycin containing plates are in the exact same position.  This demonstrates

#8. Inheritance Biology
  1. how to screen for environmental mutagens

  2. that mutations occur in prokaryotes as well as in eukaryotes

  3. that in some cases mutations are caused by the selective agent itself

  4. a direct correlation between the amount of the selective agent used and the number of resistant mutants (one-hit relationship)

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TLS Online TPP Program

#Question id: 12723

#6. System Physiology – Plant

GroEL, GroES is an example of the  prokaryotic cell, this HSP protein is belong to the which of the following HSP class?

TLS Online TPP Program

#Question id: 12724

#6. System Physiology – Plant

In response to sudden, 5 to 10°C rises in temperature, plants produce a unique set of proteins referred to as heat shock proteins (HSPs). There are five classes of heat shock proteins found in plants such as;

       HSP class

 Examples (Arabidopsis / prokaryotic)

      Cellular location

 

I) HSP100

 

 

a) AtTCP-1 / GroEL, GroES

 

i) Cytosol

 

II) HSP90

 

 

b) Various AtHSP22, AtHSP20, AtHSP18.2, AtHSP17.6 / IBPA/B

 

ii) mitochondria

 

III) HSP70

 

 

c) AtHSP101 / ClpB, ClpA/C

 

iii) chloroplasts

 

IV) HSP60

 

 

d) AtHSP70 / DnaK

 

iv) endoplasmic reticulum

 

V) smHSP

 

 

e) AtHSP90 / HtpG

 

Match the following HSPs   with their correct location and the examples of the HSPs;


TLS Online TPP Program

#Question id: 12740

#13. Methods in Biology

Choose the incorrect statement for Agglutination.

TLS Online TPP Program

#Question id: 12741

#13. Methods in Biology

 What will happen if too much antibody is present in the Agglutination process?

TLS Online TPP Program

#Question id: 12742

#13. Methods in Biology

What will happen if too much antigen is present in the Agglutination process?

TLS Online TPP Program

#Question id: 12743

#13. Methods in Biology

Name the agar plate method which is most common gel based assay system used in Agglutination process.