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TLS Online TPP Program

#Question id: 8845

The experimental approach taken in current biological investigations presumes that

#Unit 9. Diversity of Life Forms
  1. simple organic compounds can be synthesized in the laboratory from inorganic precursors, but complex organic compounds like carbohydrates and proteins can only be synthesized by living organisms.
  2. living organisms can be understood in terms of the same physical and chemical laws that can be used to explain all natural phenomena.
  3. although a life force, or vitalism, exists in living organisms, this life force cannot be studied by physical or chemical methods.
  4. living organisms are composed of the same elements present in nonliving things, plus a few special trace elements found only in living organisms or their products. 
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TLS Online TPP Program

#Question id: 19790

#Unit 13. Methods in Biology

What will be the major difference between the PCR and replication is?
TLS Online TPP Program

#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?
a) PCR denaturation temperature calculated by using Y = X (1+E)n
b) Size of the primer negatively affects the efficiency of PCR
c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.
d) Primer annealing of PCR at 550C for 1.5 min.
e) Time taken for each cycle of PCR considerably longer than 3 min.
TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

                 DNA Polymerase

                        Sources

 

 A- Tma

 

i) Pyrococcus furiosus

 

 B- Deep VentTM

 

ii) Pyrococcus woesi

 

 C- Tli

 

iii)  Thermotoga maritima

 

 D- Pfu

 

iv) Pyrococcus sp.

 

 E- Pwo

 

v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?
TLS Online TPP Program

#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___
a) Inter-complemetary primers
b) Hybrid 
c) Amplicons
TLS Online TPP Program

#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__
a) Denaturation
b) Annealing
c) Phosphorylation
d) Amplification
e) Methylation
f) Extension
Which of the following steps are correctly analyzed by real time PCR?