Practice Questions

TLS Online TPP Program

#Question id: 7296

some of the following statements regarding to this experiment are given below. Which of the following statements is incorrect?

#Unit 5. Developmental Biology

If a small piece of tissue from a plant stem or leaf is placed on a solid agar medium containing the appropriate nutrients and growth hormones, the cells will start to divide to form a disorganized mass of cells known as a callus.
If a small piece of tissue from a plant stem or leaf is placed on a solid agar medium, it does not need any nutrients and growth hormones, the cells will start to divide to form a disorganized mass of cells known as a callus.
The callus cells are then separated and grown in liquid culture, again containing the appropriate growth hormones
In suspension culture, some of the callus cells divide to form small cell clusters. These cell clusters can resemble the globular stage of a dicotyledon embryo, and with further culture on solid medium, develop through the heart shaped and later stages to regenerate a complete new plant.

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TLS Online TPP Program

#Question id: 19789

#Unit 13. Methods in Biology

How to determine the size of PCR product?

TLS Online TPP Program

#Question id: 19790

#Unit 13. Methods in Biology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase

Sources

A- Tma

i) Pyrococcus furiosus

B- Deep Vent^TM

ii) Pyrococcus woesi

C- Tli

iii) Thermotoga maritima

D- Pfu

iv) Pyrococcus sp.

E- Pwo

v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?

TLS Online TPP Program

#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___

a) Inter-complemetary primers