Practice Questions

TLS Online TPP Program

#Question id: 11131

Which statement concerning the generation of an action potential is correct?

#Unit 7. System Physiology – Animal

When the membrane potential in the soma/axon hillock dips below “threshold,” an action potential is initiated
The action potential is initiated in synaptic boutons
The least number of voltage-gated sodium channels in an axon is found near the node of Ranvier
Once an action potential is initiated, it will always run its course to completion

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TLS Online TPP Program

#Question id: 19789

#Unit 13. Methods in Biology

How to determine the size of PCR product?

TLS Online TPP Program

#Question id: 19790

#Unit 13. Methods in Biology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase

Sources

A- Tma

i) Pyrococcus furiosus

B- Deep Vent^TM

ii) Pyrococcus woesi

C- Tli

iii) Thermotoga maritima

D- Pfu

iv) Pyrococcus sp.

E- Pwo

v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?

TLS Online TPP Program

#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___

a) Inter-complemetary primers