In Drosophila melanogaster, vestigial wings are determined by a recessive allele of a gene that is linked to a gene with a recessive allele that determines black body color. T. H. Morgan crossed black-bodied, normal-winged females and gray-bodied, vestigial-winged males. The F1 were all gray bodied, normal winged. The F1 females were crossed to homozygous recessive males to produce testcross progeny. Morgan calculated the map distance to be 17 map units. Which of the following information is correct about the testcross progeny?

 Group (family name)

                           Functional information

I) Group 1

   (D-19 family)

a) It is an abundant protein in cotton embryos, Each putative dimer of this group may bind as many as ten inorganic phosphates and their counterions

II) Group 2

    (D-11 family)

b) Possibly functions in ion sequestration to protect cytosolic metabolism When LE25 is expressed in yeast, it confers salt and freezing tolerance

III) Group 3

     (D-7 family)

c) In tomato, a gene encoding a similar protein is expressed in response to nematode feeding

IV) Group 4

    (D-95 family)

d) Contains more water of hydration than typical globular proteins Overexpression confers water deficit tolerance on yeast cells

V) Group 5

    (D-113 family)

e) Often localized to the cytoplasm or nucleus More acidic members of the family are associated with the plasma membrane May act to stabilize macromolecules at low water potential

       HSP class

 Examples (Arabidopsis / prokaryotic)

      Cellular location

I) HSP100

a) AtTCP-1 / GroEL, GroES

i) Cytosol

II) HSP90

b) Various AtHSP22, AtHSP20, AtHSP18.2, AtHSP17.6 / IBPA/B

ii) mitochondria

III) HSP70

c) AtHSP101 / ClpB, ClpA/C

iii) chloroplasts

IV) HSP60

d) AtHSP70 / DnaK

iv) endoplasmic reticulum

V) smHSP

e) AtHSP90 / HtpG