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QUESTION ID:1

In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by

QUESTION ID:2

The basis for blue-white screening with pUC vectors is

QUESTION ID:3

Idiotypic determinants of an antibody are associated with the

QUESTION ID:4

I dentification of blood groups involves

QUESTION ID:5

B -lymphocytes originate from the bone marrow whereas T-lymphocytes originate from

QUESTION ID:6

A humanized antibody is one in which the

QUESTION ID:7

Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

QUESTION ID:8

Nude mice refers to

QUESTION ID:9

Heat inactivation of serum is done to inactivate

QUESTION ID:10

Choose the correct signal transduction pathway.

QUESTION ID:11

A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with

QUESTION ID:12

A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?

QUESTION ID:13

Protein-DNA interactions in vivo can be studied by

QUESTION ID:14

The direction of shell coiling in the snail Limnaea peregra is a classic example of

QUESTION ID:15

During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose 1,5- bisphosphate to yield

QUESTION ID:16

Which one of the following is NOT a protoplast fusion inducing agent?

QUESTION ID:17

The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is

QUESTION ID:18

Identify the statement that is NOT applicable to an enzyme catalyzed reaction.

QUESTION ID:19

An example of a derived protein structure database is

QUESTION ID:20

An example of a program for constructing a phylogenetic tree is

QUESTION ID:21

Synteny refers to

QUESTION ID:22

W hile searching a database for similar sequences, E value does NOT depend on the

QUESTION ID:23

In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with

QUESTION ID:24

The molarity of water in a water : ethanol mixture (15 : 85, v/v) is approximately

QUESTION ID:25

The helix content of a protein can be determined using

QUESTION ID:26

Which one of the following DNA sequences carries an invert repeat?

QUESTION ID:27

In zinc finger proteins, the amino acid residues that coordinate zinc are

QUESTION ID:28

Match the entries in Group I with those in Group II.

Group IGroup II
P. MTT1. Dihydrofolate reductase
Q. Annexin V2. Succinate dehydrogenase
R. Methotrexate3. Microtubules
S. Taxol4. Phosphatidylserine

QUESTION ID:29

In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 20 gl^-1 (DCW), the specific growth rate is 0.4 h^-1 and substrate uptake rate (v) is 16 gl^-1h^-1. The cell yield coefficient Yx/s will be

QUESTION ID:30

A single base pair of DNA weighs 1.1 × 10^-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 of purified DNA?

QUESTION ID:31

Match the terms in Group I with the ploidy in Group II.

Group IGroup II
P. Disome1. 2n + 1
Q. Monosome2. 2n - 1
R. Nullisome3. n - 1
S. Trisome4. n + 1

QUESTION ID:32

What is the rank of the following matrix?


QUESTION ID:33

Match the products in Group I with the applications in Group II.

Group IGroup II
P. Digoxin1. Muscle relaxant
Q. Stevioside2. Anti-cancer agent
R. Atropine3. Cardiovascular disorder
S. Vinblastine4. Sweetener

QUESTION ID:34

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: The production of secondary metabolites in plant cell cultures is enhanced by the addition of elicitors.
Reason: Elicitors induce the expression of enzymes responsible for the biosynthesis of secondary metabolites.

QUESTION ID:35

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: Plants convert fatty acids into glucose.
Reason: Plants have peroxisomes.

QUESTION ID:36

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: In direct somatic embryogenesis, embryos are developed without going through callus formation.
Reason: This is possible due to the presence of pre-embryonically determined cells.

QUESTION ID:37

Match the entries in Group I with the process parameters in Group II.

Group IGroup II
P. Clark electrode1. Liquid level
Q. Redox probe2. Dissolved oxygen concentration
R. Load cell3. Vessel pressure
S. Diaphragm gauge4. pH (anaerobic process)

QUESTION ID:38

Match the downstream processes in Group I with the products in Group II

Group IGroup II
P. Solvent extraction                                    1. Lactic acid  
Q. Protein-A linked affinity chromatography2. Penicillin
R. Extractive distillation3. Monoclonal antibody
S. Salting out4. Lipase                                    

QUESTION ID:39

Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
Assertion: Cell mass yield of a methylotrophic yeast is more on methanol compared to glucose.
Reason: Methanol has a greater degree of reductance compared to glucose.

QUESTION ID:40

Adisease is inherited by a child with a probability of 1/4. In a family with two children, the probability that exactly one sibling is affected by this disease is

QUESTION ID:41

Match the organisms in Group I with the entries in Group II.

Group IGroup II
P. Clostridium1. Rods with teichoic acid in the cell wall
Q. Escherichia2. Rods with endospores
R. Vibrio3. Helical rods with flagella
S. Bacillus4. Rods with LPS in the outer membrane
5. Curved rods with polar flagella

QUESTION ID:42

Match the entries in Group I with the methods of sterilization in Group II.

Group IGroup II
P. Serum1. Autoclave
Q. Luria broth2. Membrane filtration
R. Polypropylene tubes3. UV irradiation
S. Biological safety cabinets4. Gamma irradiation
5. Dry heat

QUESTION ID:43

Match the high energy compounds in Group I with the biosynthetic pathways for the molecules in Group II.

Group IGroup II
P. GTP1. Fatty acid
Q. UTP2. Phospholipid  
R. CTP                    3. Protein
S. Acyl coenzyme A4. Peptidoglycan

QUESTION ID:44

Match the vitamins in Group I with the processes/reactions in Group II.

Group IGroup II
P. Pantothenic acid1. Electron transport
Q. Vitamin B22. Transfer of 1-C units
R. Vitamin B63. Decarboxylation
S. Folic acid4. Fatty acid metabolism
5. Hydrolysis

QUESTION ID:45

Consider the data set 14, 18, 14, 14, 10, 29, 33, 31, 25. If you add 20 to each of the values, then

QUESTION ID:46

An enzymatic reaction is described by the following rate expression

Which one of the following curves represents this expression?


QUESTION ID:47

A bacterial culture (200 containing 1.8 × 10^9 cells) was treated with an antibiotic Z (50 per ml) for 4 h at 37˚C. After this treatment, the culture was divided into two equal aliquots.
Set A: 100 was plated on Luria agar.
Set B: 100 was centrifuged, the cell pellet washed and plated on Luria agar.
After incubating these two plates for 24 h at 37˚C, Set A plate showed no colonies, whereas the Set B plate showed 0.9 × 10^9 cells. This experiment showed that the antibiotic Z is

QUESTION ID:48

In a muscle, the extracellular and intracellular concentrations of Na^+ are 150 mM and 12 mM, and those of K^+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25^oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol^-1 K^-1; F = 96.45 kJ mol^-1 V^-1)
The free energy change for the transport of three Na^+ out of the cell is

QUESTION ID:49

In a muscle, the extracellular and intracellular concentrations of Na^+ are 150 mM and 12 mM, and those of K^+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25^oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol^-1 K^-1; F = 96.45 kJ mol^-1 V^-1)

The free energy change for the transport of two K^+ into the cell is

QUESTION ID:50

The purification data for an enzyme is given below:

The fold purification for each step is

QUESTION ID:51

The purification data for an enzyme is given below:

The yield (%) for each step is

QUESTION ID:52

An E. coli cell of volume 10^-12 cm^3 contains 60 molecules of lac-repressor. The repressor has a binding affinity (Kd) of 10^-8 M and 10^-9 M with and without lactose respectively, in the medium. The molar concentration of the repressor in the cell is

QUESTION ID:53

An E. coli cell of volume 10^-12 cm^3 contains 60 molecules of lac-repressor. The repressor has a binding affinity (Kd) of 10^-8 M and 10^-9 M with and without lactose respectively, in the medium. Therefore the lac-operon is

QUESTION ID:54

Beta-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume 100 liters and a voidage of 0.55. The K'm and V'max for the immobilized enzyme are 0.72 gl^-1 and 18 gl^-1h^-1, respectively. The lactose concentration in the field stream is 20 gl^-1, and a fractional conversion of 0.90 is desired. Diffusional limitations may be ignored.
The residence time required for the steady state reactor operation will be

QUESTION ID:55

Beta-Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose and galactose in a plug flow bioreactor with a packed bed of volume 100 liters and a voidage of 0.55. The K'm and V'max for the immobilized enzyme are 0.72 gl^-1 and 18 gl^-1h^-1, respectively. The lactose concentration in the field stream is 20 gl^-1, and a fractional conversion of 0.90 is desired. Diffusional limitations may be ignored.

The feed flow rate required for the above bioconversion will be

QUESTION ID:56

The cost function for a product in a firm is given by 5q^2, where q is the amount of production. The firm can sell the product at a market price of 50 per unit. The number of units to be produced by the firm such that the profit is maximized is

QUESTION ID:57

Choose the most appropriate alternative from the options given below to complete the following sentence:
Suresh’s dog is the one ––––––––– was hurt in the stampede.

QUESTION ID:58

hoose the grammatically INCORRECT sentence:

QUESTION ID:59

Which one of the following options is the closest in meaning to the word given below?
Mitigate

QUESTION ID:60

Choose the most appropriate alternative from the options given below to complete the following sentence:
Despite several ––––––––– the mission succeeded in its attempt to resolve the conflict.

QUESTION ID:61

Wanted Temporary, Part-time persons for the post of Field Interviewer to conduct personal interviews to collect and collate economic data. Requirements: High School-pass, must be available for Day, Evening and Saturday work. Transportation paid, expenses reimbursed.
Which one of the following is the best inference from the above advertisement?

QUESTION ID:62

Given the sequence of terms, AD CG FK JP, the next term is

QUESTION ID:63

Which of the following assertions are CORRECT?
P: Adding 7 to each entry in a list adds 7 to the mean of the list
Q: Adding 7 to each entry in a list adds 7 to the standard deviation of the list
R: Doubling each entry in a list doubles the mean of the list
S: Doubling each entry in a list leaves the standard deviation of the list unchanged

QUESTION ID:64

An automobile plant contracted to buy shock absorbers from two suppliers X and Y. X supplies 60% and Y supplies 40% of the shock absorbers. All shock absorbers are subjected to a quality test. The ones that pass the quality test are considered reliable. Of X’s shock absorbers, 96% are reliable. Of Y’s shock absorbers, 72% are reliable.
The probability that a randomly chosen shock absorber, which is found to be reliable, is made by Y is

QUESTION ID:65

A political party orders an arch for the entrance to the ground in which the annual convention is being held. The profile of the arch follows the equation y = 2x – 0.1x^2 where y is the height of the arch in meters. The maximum possible height of the arch is