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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1


QUESTION ID:2

If an unbiased coin is tossed 10 times, the probability that all outcomes are same will be ___ x10^-5

QUESTION ID:3

The solution for the following set of equations is


QUESTION ID:4

The limit of the function 𝑒^−2𝑡 sin(t) as t → ∞, is----

QUESTION ID:5

The solution to the following set of equations is

2x+3y = 4

4x+6y = 0

QUESTION ID:6

The unit for specific substrate consumption rate in a growing culture is 

QUESTION ID:7

If the dissociation cakilitZr solute-adsorbent binding is KD, the retention time of the solute in a chromatography column 

QUESTION ID:8

In a batch culture of Penicillium chrysogenum, the maximum penicillin synthesis occurs during the

QUESTION ID:9

The most plausible explanation for a sudden increase of the respiratory quotient (RQ) of a microbial culture is that

QUESTION ID:10

Which of the following is employed for the repeated use of enzymes in bioprocesses?

QUESTION ID:11

Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given N = rotations/time, D=diameter of impeller)

QUESTION ID:12

The degree of reduction of ethanol is _______

QUESTION ID:13

Gram-positive bacteria are generally resistant to complement-mediated lysis because

QUESTION ID:14

A bacterium belonging to cocci group has a diameter of 2 μm. The numerical value of the ratio of its surface area to volume (in μm−1) is ________

QUESTION ID:15

Which of the following essential element(s) is/are required as major supplement to enhance the bioremediation of oil spills by the resident bacteria?

QUESTION ID:16

The 4- amino or 4- Keto group of pyrimidine bases is located in the

QUESTION ID:17

The product(s) resulting from the hydrolysis of maltose is/are

QUESTION ID:18

Amino acid residue which is most likely to be found in the interior of water-soluble globular proteins is

QUESTION ID:19

The 5’ ends of the mature forms of the prokaryotic mRNAs and tRNAs are

QUESTION ID:20

Prior exposure of plants to pathogens is known to increase resistance to future pathogen attacks. This phenomenon is known as

QUESTION ID:21

Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel are referred as

QUESTION ID:22

The algorithm for BLAST is based on

QUESTION ID:23

The statistical frequency of the occurrence of a particular restriction enzyme cleavage site that is 6 bases long can be estimated to be

QUESTION ID:24

The reactions leading to the formation of amino acids from the TCA cycle intermediates are

QUESTION ID:25

The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to

QUESTION ID:26

The concentration profile of a chemical at a location x and time t, denoted by 𝑐𝑐(𝑥𝑥, 𝑡𝑡), changes as per the following equation,
where 𝐷 and 𝑐0 are assumed to be constant. Which of the following is correct?

QUESTION ID:27


QUESTION ID:28

Which of the following statements is true for the series given below?

QUESTION ID:29

The graph of the function 

QUESTION ID:30

A T-flask is seeded with 10^5 anchorage-dependent cells. The available area of the T-flask is 25 cm^2 and the volume of the medium is 25 ml. Assume that the cells are rectangles of size 5 μm × 2 μm. If the cells grow to monolayer confluence after 50 h, the growth rate in number of cells/(cm^2.h) is ____ x10^5

QUESTION ID:31

Consider a continuous culture provided with a sterile feed containing 10 mM glucose. The steady state cell density and substrate concentration at three different dilution rates are given in the table below.
The maximum specific growth rate μm (in h^−1), will be _____.

QUESTION ID:32

Cholera toxin increases cAMP levels by

QUESTION ID:33

Triose phosphate isomerase converts dihydroxy acetone phosphate (DHAP) to glyceraldehyde-3- phosphate (G-3-P) in a reversible reaction. At 298 K and pH 7.0, the equilibrium mixture contains 40 mM DHAP and 4 mM G-3-P. Assume that the reaction started with 44 mM DHAP and no G-3-P. The standard free-energy change in kJ/mol for the formation of G-3-P [R = 8.315 J/mol.K] is _________.

QUESTION ID:34

The plasmid DNA was subjected to restriction digestion using the enzyme EcoRI and analysed on an agarose gel. Assuming digestion has worked (the enzyme was active), match the identity of the DNA bands shown in the image in Group I with their identity in Group II

QUESTION ID:35

In a relatively large but finite and closed population of sexually reproducing diploid organisms, the frequency of homozygous genotype PP changes from 0.40 to 0.50 and that of pp changes from 0.40  to 0.41 in a span of 10 generations. Which of the following is the most likely cause for the above change in frequency of the PP genotype?

QUESTION ID:36

Topological winding number of a 2.0 kb covalently closed circular DNA was found to be 191 with a writhing number of -4. Hence, its LINKING NUMBER and the NUMBER OF BASE PAIR PER TURN when the molecule is laid flat on the surface is _______ and_________, respectively.

QUESTION ID:37

Consider a population of 10,000 individuals, of which 2500 are homozygotes (PP) and 3000 are heterozygotes (Pp) genotype. The frequency of allele p in the population is _________ .

QUESTION ID:38

Match the following photoreceptors with their prosthetic groups and spectral specificity


QUESTION ID:39

Match the following plant sources with their secondary metabolites and medical uses


QUESTION ID:40

The pungency of mustard seeds is primarily due to secondary metabolites such as isothiocyanate and nitrile. The pungency is usually felt only when the seeds are crushed. This is because of

QUESTION ID:41

In a mouse genome, the numbers of functional gene segments are 79, 38, 21, 2 and 11, respectively. The total number of possible combinations for αβ T cell receptors are _____x10^6.

QUESTION ID:42

The percentage SIMILARITIES and IDENTITIES, respectively, between the two peptide sequences given below will be ______% and _____%.

QUESTION ID:43

In an affine gap penalty model, if the gap opening penalty is -20, gap extension penalty is -4 and gap length is 8, the gap score is _________.

QUESTION ID:44

For their efficient translation, eubacterial mRNAs possess a Shine-Dalgarno sequence for its recognition by an anti-Shine-Dalgarno sequence (ASD) in the ribosomes. The correct statement is

QUESTION ID:45

Match the items in Group I with Group II


QUESTION ID:46

Match the immunoglobulin class in Group I with its properties in Group II


QUESTION ID:47

In a genetic cross between the genotypes WWXX and wwxx, the following phenotypic distributions were observed among the F2 progeny: WX, 562; wx, 158; Wx, 38; and wX, 42. Likewise, a cross between XXYY and xxyy yielded the following results: XY, 675; xy, 175; Xy, 72; and xY, 78. Similarly, a cross between WWYY and wwyy yielded: WY, 292; wy, 88; Wy, 12; and wY, 8. In all the genotypes, capital letters denote the dominant allele. Assume that the F1 progeny were self-fertilized in all three crosses. Also, double cross-over does not occur in this species. Which of the following is correct?

QUESTION ID:48

The length of the minimum unique stretch of DNA sequence that can be found only once in a 3 billion base pairs long genome is

QUESTION ID:49

Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was gradually decreasing. Microbiological assays of reactor samples showed absence of contamination and recorded data showed no change in the operating conditions. The most probable reason for decrease in lysine concentration may be attributed to

QUESTION ID:50

If a plant is shifted to cold temperature, which of the following changes would take place in its membrane?

QUESTION ID:51

If protoplasts are placed in distilled water, they swell and burst as a result of endosmosis. The plot representing the kinetics of burst is

QUESTION ID:52

Which of the following statements with respect to the orientation of the nitrogenous bases to the pentose sugars, and the puckering of the sugar, is correct?

QUESTION ID:53

A dioecious plant has XX sexual genotype for female and XY for male. After double fertilization, what would be the genotype of the embryos and endosperms?

QUESTION ID:54

The amino acid substitution matrices in decreasing order of stringency for comparing protein sequences are

QUESTION ID:55

The active site in the alpha/beta barrel structures is usually located

QUESTION ID:56

Choose the most appropriate word from the options given below to complete the following sentence.
A person suffering from Alzheimer’s disease ......... short-term memory loss.

QUESTION ID:57

Choose the most appropriate word from the options given below to complete the following sentence.
____________ is the key to their happiness; they are satisfied with what they have.

QUESTION ID:58

Which of the following options is the closest in meaning to the sentence below?
“As a woman, I have no country.”

QUESTION ID:59

In any given year, the probability of an earthquake greater than Magnitude 6 occurring in the Garhwal Himalayas is 0.04. The average time between successive occurrences of such earthquakes is ____ years

QUESTION ID:60

The population of a new city is 5 million and is growing at 20% annually. How many years would it take to double at this growth rate?

QUESTION ID:61

In a group of four children, Som is younger to Riaz. Shiv is elder to Ansu. Ansu is youngest in the group. Which of the following statements is/are required to find the eldest child in the group?
Statements
1. Shiv is younger to Riaz.
2. Shiv is elder to Som.

QUESTION ID:62

Moving into a world of big data will require us to change our thinking about the merits of exactitude. To apply the conventional mindset of measurement to the digital, connected world of the twenty-first century is to miss a crucial point. As mentioned earlier, the obsession with exactness is an artefact of the information-deprived analog era. When data was sparse, every data point was critical, and thus great care was taken to avoid letting any point bias the analysis. From “BIG DATA” Viktor Mayer-Schonberger and Kenneth Cukier
The main point of the paragraph is:

QUESTION ID:63

The total exports and revenues from the exports of a country are given in the two pie charts below. The pie chart for exports shows the quantity of each item as a percentage of the total quantity of exports. The pie chart for the revenues shows the percentage of the total revenue generated through export of each item. The total quantity of exports of all the items is 5 lakh tonnes and the total revenues are 250 crore rupees. What is the ratio of the revenue generated through export of Item 1 per kilogram to the revenue generated through export of Item 4 per kilogram?

QUESTION ID:64

X is 1 km northeast of Y. Y is 1 km southeast of Z. W is 1 km west of Z. P is 1 km south of W. Q is 1 km east of P. What is the distance between X and Q in km?

QUESTION ID:65

10% of the population in a town is HIV^+. A new diagnostic kit for HIV detection is available; this kit correctly identifies HIV^+ individuals 95% of the time, and HIV− individuals 89% of the time. A particular patient is tested using this kit and is found to be positive. The probability that the individual is actually positive is _______