Menu

Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

The Bt toxin gene from Bacillus thuringiensis used to generate genetically modified crops iS

QUESTION ID:2

Which one of the following is used as a pH indicator in animal cell culture medium?

QUESTION ID:3

Tetracycline inhibits the

QUESTION ID:4

Which one of the following is a database of protein sequence motifs?

QUESTION ID:5

Which one of the following enzymes is encoded by human immunodeficiency virus (HIV) genome?

QUESTION ID:6

DNA synthesis in eukruyotes occurs during which phase of the mitotic cell cycle?

QUESTION ID:7

Match the human diseases in Group I with the causative agents in Group II. 

QUESTION ID:8

Which one of the following techniques can be used to compare the expression of a large nunber of genes in two biological samples in a single experiment?

QUESTION ID:9

Which of the following processes can increase genetic diversity of bacteria in nature?
P. Conjugation
Q. Transfonnation
R. Transduction
S. Transfection

QUESTION ID:10

Which one of the following is NOT a pru1 of the human nonspecific defense system?

QUESTION ID:11

A mutation in a gene that codes for a polypeptide results in a vru·iant polypeptide that lacks the last three amino acids. What type of mutation is this?

QUESTION ID:12

Which one of the following equations represents a one-dimensional wave equation?


QUESTION ID:13

Which of the following are geometric series?
P. 1, 6, 11, 16, 21, 26, ...
Q. 9, 6, 3, 0, -3, -6, .. .
R. 1, 3, 9, 27, 81, .. .
S. 4, -8, 16, -32, 64, ...

QUESTION ID:14

Which one of the following statements is CORRECT for enzyme catalyzed reactions? ( is Gibbs free energy change, Keq is equilibrium constant)

QUESTION ID:15

Which one of the following can NOT be a limiting substrate ifMonod's growth kinetics is applicable?

QUESTION ID:16

Which one of the following is the unit of heat transfer coefficient?

QUESTION ID:17

Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?

QUESTION ID:18

Which one of the following need NOT be conse1ved in a biochemical reaction?

QUESTION ID:19

The number of possible rooted trees in a phylogeny of three species is ___ _

QUESTION ID:20

Matrix will be skew-symmetric when p = __ .

QUESTION ID:21

The soulution    ____ _

QUESTION ID:22

The median value for the dataset (12, 10, 16, 8, 90, 50, 30, 24) is ____ _

QUESTION ID:23

The degree of reduction for acetic acid is ___ _

QUESTION ID:24

The mass of 1 kmol of oxygen molecules is ___ g (rounded off to the nearest integer).

QUESTION ID:25

Protein concentration of a cmde enzyme preparation was 10 mg mL^-1. 10 of this sample gave an activity of 5 under standard assay conditions. The specific activity of this cmde enzyme preparation is units mg^-1.

QUESTION ID:26

In general, which one of the following statements is NOT CORRECT?

QUESTION ID:27

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?

QUESTION ID:28

Match the organelles in Group I with their fimctions in Group II.


QUESTION ID:29

Which of the following statements are CORRECT when a protein sequence database is searched using the BLAST algorithm?
P. A larger E-value indicates higher sequence similarity
Q. E-value < 10-10 indicates sequence homology
R. A higher BLAST score indicates higher sequence similarity
S. E-value > 1010 indicates sequence homology

QUESTION ID:30

Which one of the following is coded by the ABO blood group locus in the human genome?

QUESTION ID:31

Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg^2+ concentration
Q. pH
R. Annealing temperature

QUESTION ID:32

Group I lists spectroscopic methods and Group II lists biomolecular applications of these methods. Match the methods in Group I with the applications in Group II.

QUESTION ID:33

The hexapeptide P has an isoelectric point (pI) of 6.9. Hexapeptide Q is a variant ofP that contains valine instead of glutamate at position 3. The two peptides are analyzed by polyacrylamide gel electrophoresis at pH 8.0. Which one of the following statements is CORRECT?

QUESTION ID:34

Antibody-producing hybridoma cells are generated by the fusion of a

QUESTION ID:35

Which of the following statements are CORRECT about the function of fetal bovine semm in animal cell culture?
P. It stimulates cell growth
Q. It enhances cell attachment
R. It provides h01mones and minerals
S. It maintains pH at 7.4

QUESTION ID:36

Which one of the following covalent linkages exists between 7-Methyl guanosine (m^7G) and mRNAs?

QUESTION ID:37

Which one of the following amino acid residues will destabilize an a-helix when inselied in the middle of the helix?

QUESTION ID:38

What is the solution of the differential equation , with the initial condition, at x = 0, y = 1?


QUESTION ID:39

The Laplace transf01m of the function f(t) = t ^2 + 2t + 1 is


QUESTION ID:40

Which of the following factors can influence the lag phase of a microbial culture in a batch fetmentor?
P. Inoculum size
Q. Inoculum age
R. Medium composition

QUESTION ID:41

Which one of the following statements is CORRECT about proportional controllers?

QUESTION ID:42

Match the instnunents in Group I with their conesponding measmements in Group II.


QUESTION ID:43

Which of the following statements is ALWAYS CORRECT about an ideal chemostat?
P. Substrate concentration inside the chemostat is equal to that in the exit stream
Q. Optimal dilution rate is lower than critical dilution rate
R. Biomass concentration increases with increase in dilution rate
S. Cell recirculation facilitates operation beyond critical dilution rate

QUESTION ID:44

Detennine the conectness or othetwise of the following Assettion [a] and the Reason [r]
Assettion [a]: It is possible to regenerate a whole plant from a single plant cell.
Reason [r]: It is easier to introduce transgenes in to plants than animals.

QUESTION ID:45

A UV-visible specu·ophotometer has a minimum detectable absorbance of 0.02. The minimum concenu·ation of a protein sample that can be measured reliably in this instrument with a cuvette of 1 cm path length is 11M. The molar extinction coefficient of the protein is 10,000 L mol^-1 cm^-1.

QUESTION ID:46

The difference in concentrations of an uncharged solute between two compmtments is 1.6-fold. The energy required for active u·anspmt of the solute across the membrane sepm·ating the two compattments is __ cal mol^-1 (rounded off to the nem·est integer). (R = 1.987 cal mol^-1 K-^1 T = 37 °C)

QUESTION ID:47

In pea plants, purple color of flowers is detennined by the dominant allele while white color is detemrined by the recessive allele. A genetic cross between two pmple flower  bearing plants results in an offspring with white flowers. The probability that the third offspring from these parents will have pmple flowers is (rounded off to 2 decimal places).

QUESTION ID:48

The moleculm· mass of a protein is 22 kDa. The size of the eDNA (excluding the untranslated regions) that codes for this protein is kb (rounded off to 1 decimal place).

QUESTION ID:49

A new game is being introduced in a casino. A player can loseRs. 100, break even, win Rs. 100, or win Rs. 500. The probabilities (P(X)) of each of these outcomes (X) are given in the following table:
The standard deviation for the casino payout is Rs. ____ (rounded off to the nearest integer).

QUESTION ID:50

 calculated using trapezoidal mle for the values given in the table is ____ (rounded off to 2 decimal places).

QUESTION ID:51

Yeast biomass grown on glucose is described by the stoichiometric equation given below:
The amount of glucose needed for the production of 50 g L^-1 of yeast biomass in a batch reactor with a working volume of 1,00,000 L is kg (rounded off to the nearest integer).

QUESTION ID:52

Phenolic wastewater discharged from an industry was n·eated with Pseudomonas sp. in an aerobic bioreactor. The influent and effluent concenn·ations of phenol were 10,000 and 10 ppm, respectively. The inlet feed rate of wastewater was 80 L h^-1. The kinetic propetties of the organism are as follows:
Maximum specific growth rate = 1 h^-1
Saturation constant = 100 mg L-1
Cell death rate (kd) = 0.01 h-1
Assuming that the bioreactor operates under 'chemostat' mode, the working volume required for this process is L (rounded off to the nearest integer).

QUESTION ID:53

In a cross-flow filn·ation process, the pressure drop driving the fluid flow is 2 ann, inlet feed pressure (Pi) is 3 ann and filn·ate pressure (P1) is equal to annospheric pressure. The average transmembrane pressure drop is atm

QUESTION ID:54

An indusntrial fetmentor containing 10,000 L of medium needs to be sterilized. The initial spore concenn·ation in the medium is 10^6 spores mL^-1. The desired probability of contamination after sterilization is 10-^3. The death rate of spores at 121^ oc is 4 min^-1. Assume that there is no cell death during heating and cooling phases. The holding time of the sterilization process is min (rounded off to the nearest integer).

QUESTION ID:55

The dimensions and operating condition of a lab-scale fe1mentor are as follows:
Volume= 1 L
Diameter = 20 em
Agitator speed = 600 rpm
Ratio of impeller diameter to fe1mentor diameter= 0.3
This fe1mentor needs to be scaled up to 8,000 L for a large scale industrial application. If the scale-up is based on constant impeller tip speed, the speed of the agitator in the larger reactor is 1pm. Assume that the scale-up factor is the cube root of the ratio of fermentor volumes.

QUESTION ID:56

The fishermen, ___ the flood victims owed their lives, were rewarded by the govemment.

QUESTION ID:57

Some students were not involved in the strike.
If the above statement is hue, which of the following conclusions is/are logically necessary?
1. Some who were involved in the strike were students.
2. No student was involved in the strike.
3. At least one student was involved in the strike.
4. Some who were not involved in the su·ike were students.

QUESTION ID:58

The radius as well as the height of a circular cone increases by 10%. The percentage increase in its volume is ---

QUESTION ID:59

Five numbers 10, 7, 5, 4 and 2 are to be ananged in a sequence from left to right following the directions given below:
1. No two odd or even numbers are next to each other.
2. The second number from the left is exactly half ofthe left-most munber.
3. The middle number is exactly twice the right-most munber.
Which is the second munber from the right?

QUESTION ID:60

Until Iran came along, India had never been _______ in kabaddi.

QUESTION ID:61

Since the last one year·, after a 125 basis point reduction in repo rate by the Reserve Bank of India, banking institutions have been making a demand to reduce interest rates on small saving schemes. Finally, the government announced yesterday a reduction in interest rates on small saving schemes to bring them on pru· with fixed deposit interest rates. Which one of the following statements can be infeued from the given passage?

QUESTION ID:62

In a cmmtry of 1400 million population, 70% own mobile phones. Among the mobile phone owners, only 294 million access the Intemet. Among these Intemet users, only half buy goods from e-commerce portals. What is the percentage of these buyers in the country?

QUESTION ID:63

The nomenclature of Hindustani music has changed over the centmi.es. Since the medieval period dhntpad styles were identified as baanis. Tenus like gayaki and baa} were used to refer to vocal and instrumental styles, respectively. With the institutionalization of music education the term gharana becrune acceptable. Gharana originally referred to hereditruy musicians from a pruticulru· lineage, including disciples and grand disciples.
Which one of the following pairings is NOT couect?

QUESTION ID:64

Two trains started at 7AM from the same point. The first train travelled n01ih at a speed of 80kmlh and the second train travelled south at a speed of 100 km/h. The time at which they were 540 km aprut is ........ AM.

QUESTION ID:65

"I read somewhere that in ancient times the prestige of a kingdom depended upon the munber of taxes that it was able to levy on its people. It was vety much like the prestige of a head-hunter in his own coommunity."
Based on the paragraph above, the prestige of a head-hunter depended upon ____ _