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Previous Year Questions

TLS Online TPP Program

QUESTION ID:1

Protein P becomes functional upon phosphorylation of a serine residue. Replacing 
this serine with                      will result in a phosphomimic mutant of P

QUESTION ID:2

 Ras protein is a 

QUESTION ID:3

Which of the following statements are CORRECT?
[P] Viruses can play a role in causing human cancer
[Q] A tumor suppressor gene can be turned off without any change in its DNA  sequence 
[R] Alteration in miRNA expression levels contributes to the development of cancer

QUESTION ID:4

 Which class of antibody is first made by developing B cells inside bone marrow? 

QUESTION ID:5

Determine the correctness or otherwise of the following. Assertion [a] and the Reason [r] regarding mammalian cells. 
Assertion [a]: Cells use Ca^2+, and not Na^+, for cell-to-cell signaling 
Reason [r]: In the cytosol, concentration of Na^+ is lower than that of Ca^+

QUESTION ID:6

Vincristine and vinblastine, two commercially important secondary metabolites from Catharanthus roseus, are examples of

QUESTION ID:7

DNA synthesized from an RNA template is called 

QUESTION ID:8

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?

QUESTION ID:9

During a positive-negative selection process, transformed animal cells expressing ...........  are killed in presence of ganciclovir in the medium. 

QUESTION ID:10

A vector derived from which one of the following viruses is used for high-frequency gnomic integration of a transgene in animal cells? 

QUESTION ID:11

Which one of the following statements about Agrobacterium plasmid is CORRECT?

QUESTION ID:12

Which of the following types of molecules act as biological catalysts?
[P] Protein
[Q] RNA 
[R] Phospholipid

QUESTION ID:13

Which one of the following media components is used to maintain pl  in mammalian cell culture? 

QUESTION ID:14

Which of the following are energy transducing membranes?

[P] Plasma membrane of bacteria

[Q] Inner membrane of chloroplasts

[R] Inner membrane of mitochondria

QUESTION ID:15

Amino acid sequences of cytochrome c and ribulose 5-phosphate epimerase from 40 organisms were chosen and phylogenetic trees were obtained for each of these two protein families. 
Determine the correctness or otherwise of the following
Assertion [a] and the Reason [r]. Assertion [a]: The two trees will not be identical
Reason [r]: The nature and frequency of mutations in the two families are different

QUESTION ID:16

A microorganism isolated from a salt-rich (salt concentration –2 M) lake was found to possess diglycerol tetraethers, with polyisoprenoid alcohol side chains, as the major lipid component of its cell membrane. The isolated organism is 

QUESTION ID:17

A function f is as follows

The functions f a continuous function when c is equal to (answer is an integer). 

QUESTION ID:18

 Given that for X = 1 and Y = 0 is  (answer is an integer)

QUESTION ID:19

The elemental composition of dry biomass of a yeast species is The contribution of carbon to the dry biomass is  % (round off to 2 decimal places).  [Given: atomic weights of H, C, N, 0, P and S are 1, 12, 14, 16, 31 and 32, respectively] 

QUESTION ID:20

Solvents A and B are completely immiscible. Solute S is soluble in both these solvents. 100 g of S was added to a container which has 2 kg each of A and B. The solute is 1.5 times more soluble in solvent A than in solvent B. The mixture was agitated thoroughly and allowed to reach equilibrium. Assuming that the solute has completely dissolved, the amount of solute in solvent A phase is ....... g

QUESTION ID:21

The number of molecules of a nucleotide of molecular weight 300 g/mol present in 10 picomoles is ............ x 10^12 (round off to 2 decimal places). 

QUESTION ID:22

To facilitate mass transfer from a gas to a liquid phase, a gas bubble of radius r is introduced into the liquid. The gas bubble then breaks into 8 bubbles of equal radius. Upon this change, the ratio of the interfacial surface area to the gas phase volume for the system changes from 3/r to 3n/r. The value of n is  

QUESTION ID:23

The largest eigenvalue of the matrix 

QUESTION ID:24

A normal random variable has mean equal to 0. and standard deviation equal to 3. The probability that on a random draw the value of this random variable is greater than 0 is ..........  (round off to 2 decimal places). 

QUESTION ID:25

A variable Y is a function oft. Given that Y(t = 0) = 1 and Y(t = 1) = 2. the interval I = [0, 1] can be approximated as   

QUESTION ID:26

A block of ice at 0 °C is supplied heat at a constant rate to convert ice to superheated steam. Which one of the following trajectories correctly represents the trend of the temperature of the system with time? Assume that the specific heat of H2O is not a function of temperature. 

QUESTION ID:27

The DNA sequence shown below is to be amplified by PCR: 
5' GCTAAGATCTGAATTTTCC TTGGGCAATAATGTAGCGC3'
3' CGATTCTAGACTTAAAAGG .AACCCGTTATTACATCGCG5' 
Which one of the following pair of primers can be used for this amplification? 

QUESTION ID:28

Which of the following statements about immune response are CORRECT?
[P] T cells are activated by antigen-presenting cells
[Q] Foreign peptides are not presented to helper T cells by Class II MHC proteins 
[R] Dendritic cells are referred to as professional antigen-presenting cells 

QUESTION ID:29

Which of the following statements are CORRECT about eukaryotic cell cycle?
[P] CDKs can phosphorylate proteins in the absence of cyclins
[Q] CDKs can be inactivated by phosphorylation
[R] Degradation of cyclins is required for cell cycle progression 
[S] CDKs are not involved in chromosome condensation

QUESTION ID:30

W, X and Y are the intermediates in a biochemical pathway as shown below: 

Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed; No: growth not observed): 

What is the order of the four complementation groups in terms of the step they block? 

QUESTION ID:31

In tomato plant, red (R) is dominant over yellow (r) for fruit color and purple (P) is dominant over green (p) for stem color. Fruit color and stem color assort independently. The number of progeny plants of different fruit/stem colors obtained from a mating are as follows: 
Red fruit, purple stem — 145
Red fruit, green stem — 184
Yellow fruit, purple stem — 66
Yellow fruit, green stem — 47 
What are the genotypes of the parent plants in this mating? 

QUESTION ID:32

Some of the cytokinins used in plant tissue culture media are given below:
[P] BAP
[Q] Zeatin
[R] Kinetin
[S] 2iP 
Which of these are synthetic analogs?

QUESTION ID:33

Carl Woese used the gene sequence of which one of the following for phylogenetic taxonomy of prokaryotes? 

QUESTION ID:34

A list of pathogens (Group I) and a list of anti-microbial agents (Group II) used to treat their infections are given below. Match the pathogens with the corresponding anti-microbial agents. 


QUESTION ID:35

Determine the correctness or otherwise of the following

Assertion [a] and the Reason [r].

Assertion [a]: Dam methylase protects E. coli DNA from phage endonucleases

Reason [r]: E. coli Dam methylase methylates the adenosine residue in the sequence "GATC" 

QUESTION ID:36

Determine the correctness or otherwise of the following

Assertion [a] and the Reason [r].

Assertion [a]: Embryonic stem cells are suitable for developing knockout mice

Reason [r]: Homologous recombination is more frequent in embryonic stem cells than that in somatic cells 

QUESTION ID:37

The schematic of a plasmid with a gap in one of the strands is shown below:

 

Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?

[P] DNA ligase

[Q] Alkaline phosphatase

[R] DNA polymerase

[S] Polynucleotide kinase

QUESTION ID:38

Match sub-cellular organelles listed in Group I with their features listed in Group II


QUESTION ID:39

Which of the following strategies are used by cells for metabolic regulation?
[P] Phosphorylation - dephosphorylation
[Q] Allostery 
[R] Feedback inhibition  

QUESTION ID:40

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r]. 
Assertion [a]: A zygote and its immediate descendant cells are unspecialized and are called totipotent 
Reason [r]: Totipotent cells retain the capacity to differentiate into only a few cell types 

QUESTION ID:41

Which of the following statements about gene therapy are CORRECT? 
[P] Affected individuals, but not their progeny, can be cured through germline gene therapy 
[Q] Affected individuals, as well as their progeny, can be cured through germline gene therapy 
[R] Affected individuals, but not their progeny, can be cured through somatic gene therapy 
[S] Affected individuals, as well as their progeny, can be cured through somatic gene therapy

QUESTION ID:42

Determine the correctness or otherwise of the following Assertion [a] and the Reason  [r]. 
Assertion [a]: A genetically engineered rice that produces beta-carotene in the rice grain is called Golden rice 
Reason [r]: Enabling biosynthesis of provitamin A in the rice endosperm gives a characteristic yellow/orange color 

QUESTION ID:43

The sequence of a 1 Mb long DNA is random. This DNA has all four bases occurring in equal proportion. The number of nucleotides, on average, between two successive EcoRI recognition site GAATTC is  

QUESTION ID:44

E. coli was grown in 15N medium for several generations. Cells were then transferred to 14N medium, allowed to grow for 4 generations and DNA was isolated immediately. The proportion of total DNA with intermediate density is  (round off to 2 decimal places). 

QUESTION ID:45

A batch reactor is inoculated with 1 g/L biomass. Under these conditions, cells exhibit a lag phase of 30 min. If the specific growth rate in the log phase is 0.00417 min^-I, the time taken for the biomass to increase to 8 g/L is min (round off to 2 decimal places). 

QUESTION ID:46

The system of linear equations cx + y = 5

3x +3y= 6

has no solution when c is equal to  

QUESTION ID:47

The amino acid sequence of a peptide is Phe-Leu-Ile-Met-Ser-Leu. The number of codons that encode the amino acids present in this peptide is given below: 
Phe: 2 codons
Leu: 6 codons
Ile: 3 codons
Met: 1 codon
Ser: 4 codons
The number of unique DNA sequences that can encode this peptide is 

QUESTION ID:48

Assume that a cell culture was started with five human fibroblast cells. Two cells did not divide even once whereas the other three cells completed three rounds of cell division. At this stage, the total number of kinetochores in all the cells put together is 

QUESTION ID:49

Growth of an organism on glucose in a chemostat is characterized by Monod model with specific growth rate = 0.45 11^-1 and Ks = 0.5 g/L. Biomass from the substrate is generated as Yxs = 0.4 g/g. The chemostat volume is 0.9 L and media is fed at 1 L/h and contains 20 g/L of glucose. At steady state, the concentration of biomass in the chemostat is .......  g/L. 

QUESTION ID:50

A function f is given as:  f(X) = 4X — X^2  The function is maximized when X is equal to 

QUESTION ID:51

An infinite series S is given as: S = 1 + 2/3 + 3/9 + 4/27 + 5/81 + (to infinity) The value of S ........  is (round off to 2 decimal places). 

QUESTION ID:52

Protein A and protein B form a covalent complex. Gel filtration chromatography of this complex showed a peak corresponding to 200 kDa. SDS-PAGE analysis of this complex, with and without beta-mercaptoethanol, showed a single band corresponding to molecular weight 50 and 25 kDa, respectively. Given that the molecular weight of protein A is 25 kDa, the molecular weight of protein B is .......  kDa . 

QUESTION ID:53

The concentrations of ATP, ADP and inorganic phosphate in a cell are 2.59, 0.73 and 2.72 mM, respectively. Under these conditions, free energy change for the synthesis of ATP at 37 °C is ...... kJ/mol (round off to 2 decimal places). 
Given: free energy change for ATP hydrolysis under standard conditions is -30.5 kJ/mol and R = 8.315 kJ/mol.K 

QUESTION ID:54

An algorithm was designed to find globins in protein sequence databases. A database which has 78 globin sequences was searched using this algorithm. The algorithm retrieved 72 sequences of which only 65 were globins. The sensitivity of this algorithm is ......  % (round off to 2 decimal places). 

QUESTION ID:55

The mitochondrial electron transfer chain oxidizes NADH with oxygen being the terminal electron acceptor. The redox potentials for the two half-reactions are given below: 
The free energy change associated with the transfer of electrons from NADH to 02 is .........  kJ/mol (round off to 2 decimal places).  Given: F = 96500 C/mol. 


QUESTION ID:56

Rajiv Gandhi Khel Ratna Award was conferred ........  Mary Kom, a six-time world champion in boxing, recently in a ceremony......  the Rashtrapati Bhawan (the President's official residence) in New Delhi. )

QUESTION ID:57

Despite a string of poor performances, the chances of K. L. Rahul's selection in the team are  

QUESTION ID:58

Select the word that fits the analogy: 
Cover : Uncover :: Associate : 

QUESTION ID:59

Hit by floods, the kharif (summer sown) crops in various parts of the country have been affected. Officials believe that the loss in production of the kharif crops can be recovered in the output of the rabi (winter sown) crops so that the country can achieve its food-grain production target of 291 million tons in the crop year 2019-20 (July-June). They are hopeful that good rains in July-August will help the soil retain moisture for a longer period, helping winter sown crops such as wheat and pulses during the November-February period. 
Which of the following statements can be inferred from the given passage? 

QUESTION ID:60

Repo rate is the rate at which Reserve Bank of India (R131) lends commercial banks, and reverse repo rate is the rate at which RBI borrows money from commercial banks. 

Which of the following statements can be inferred from the above passage? 

QUESTION ID:61

P, Q, R, S, T, U, V, and W are seated around a circular table. 1. S is seated opposite to W. II. U is seated at the second place to the right of R. III. T is seated at the third place to the left of R. 1V. V is a neighbor of S. 
Which of the following must be true? 

QUESTION ID:62

The difference between the sum of the first 2n natural numbers and the sum of the first n odd natural numbers is

QUESTION ID:63

The distance between Delhi and Agra is 233 km. A car P started travelling from Delhi to Agra and another car Q started from Agra to Delhi along the same road 1 hour after the car P started. The two cars crossed each other 75 minutes after the car Q started. Both cars were travelling at constant speed. The speed of car P was 10 km/hr more than the speed of car O. How many kilometers the car Q had travelled when the cars crossed each other? 

QUESTION ID:64

For a matrix M = [m ij]; i,j = 1,2,3,4, the diagonal elements are all zero and mij = —mji. The minimum number of elements required to fully specify the matrix is

QUESTION ID:65

The profit shares of two companies P and Q are shown in the figure. If the two companies have invested a fixed and equal amount every year, then the ratio of the total revenue of company P to the total revenue of company Q, during 2013 - 2018 is