All members of the Bcl-2 family share a close homology in up to four characteristic regions, termed the Bcl-2 homology domains

TLS Online TPP Program

#Id: 5895

#Unit 3. Fundamental Processes

The palm domain is composed of a b sheet and contains the primary elements of the catalytic site. In particular, this region of DNA polymerase binds two divalent metal ions.

TLS Online TPP Program

#Id: 5896

#Unit 3. Fundamental Processes

One metal ion reduces the affinity of the 3’-OH for its hydrogen. This generates a 3’O- that is primed for the nucleophilic attack of the a-phosphate of the incoming dNTP. 

TLS Online TPP Program

#Id: 5897

#Unit 3. Fundamental Processes

The second metal ion coordinates the negative charges of the b-phosphate and g phosphate of the dNTP and stabilizes the pyrophosphate produced by joining the primer and the incoming nucleotide.

TLS Online TPP Program

#Id: 5898

#Unit 3. Fundamental Processes

The average number of nucleotides added before a polymerase dissociates defines its processivity. Nucleotides added per unit time dissociates defines its polymerisation

The 3’->5’ exonuclease activity removes the mispaired nucleotide, and the polymerase begins again known as proofreading

TLS Online TPP Program

#Id: 5899

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 5900

#Unit 3. Fundamental Processes