Four classes of ATP-powered pumps:

P-class pumps

V-class pumps

F-class pumps

ABC Superfamily

TLS Online TPP Program

#Id: 2822

#Unit 13. Methods in Biology

In Ion-exchange chromatography Cation exchangers possess negatively charged groups and these will attract positively charged cations & Anion exchangers have positively charged groups that will attract negatively charged anions

TLS Online TPP Program

#Id: 2823

#Unit 13. Methods in Biology

Examples of commonly used ion exchangers

TLS Online TPP Program

#Id: 2824

#Unit 13. Methods in Biology

Ion exchangers often use Gradient elution than isocratic elution, while Gradient elution is based on gradient of pH and ionic strength.

TLS Online TPP Program

#Id: 2825

#Unit 13. Methods in Biology

Affinity chromatography exploits the unique property of extremely specific biological interactions to achieve separation and purification.

TLS Online TPP Program

#Id: 2826

#Unit 13. Methods in Biology

Affinity chromatography exploits the unique property of extremely specific biological interactions to achieve separation and purification

TLS Online TPP Program

#Id: 2827

#Unit 13. Methods in Biology

Affinity chromatography used for the purification of enzymes, nucleotides, nucleic acids, immunoglobulins, membrane receptors and even to whole cells and cell fragments.