Integrins typically exhibit low affinities for their ligands, with dissociation constants (Kd) between 10−6 and 10−7 mol/L.

TLS Online TPP Program

#Id: 2828

#Unit 13. Methods in Biology

Ion exchangers often use Gradient elution than isocratic elution, while Gradient elution is based on gradient of pH and ionic strength.

TLS Online TPP Program

#Id: 2829

#Unit 13. Methods in Biology

Affinity chromatography exploits the unique property of extremely specific biological interactions to achieve separation and purification.

TLS Online TPP Program

#Id: 2830

#Unit 13. Methods in Biology

Affinity chromatography used for the purification of enzymes, nucleotides, nucleic acids, immunoglobulins, membrane receptors and even to whole cells and cell fragments.

TLS Online TPP Program

#Id: 2831

#Unit 13. Methods in Biology

Principle of purification of an enzyme by affinity chromatography.

TLS Online TPP Program

#Id: 2832

#Unit 13. Methods in Biology

Examples of group-specific ligands commonly used in affinity chromatography

TLS Online TPP Program

#Id: 2833

#Unit 13. Methods in Biology

The peptide or protein (known as a ‘ tag’) can be placed at the N- or C-terminal ends and provides a means for the fusion protein to be selectively purified from the cell extract by affinity chromatography.