Heavy chain contain more constant region in compare to variable region, in contrast in the light chain variable and constant region are equal.

TLS Online TPP Program

#Id: 2806

#Unit 13. Methods in Biology

The main applications of normal-phase liquid chromatography are its use to separate

analytes that have low water solubility and those that are not amenable to reversed phase liquid chromatography.

TLS Online TPP Program

#Id: 2807

#Unit 13. Methods in Biology

The pH of the buffer selected as eluent should be at least one pH unit above or below the isoionic point of the analytes.

TLS Online TPP Program

#Id: 2808

#Unit 13. Methods in Biology

Reversed-phase liquid chromatography form of liquid chromatography, which has many similarities with hydrophobic interaction chromatography (HIC).

TLS Online TPP Program

#Id: 2809

#Unit 13. Methods in Biology

In RPLC the stationary phase is non-polar and the mobile phase relatively polar, hence the name reversed-phase

TLS Online TPP Program

#Id: 2810

#Unit 13. Methods in Biology

In RPLC stationary phase most commonly used is in the bonded-phase form, in which alkylsilane groups are chemically attached to silica. Butyl (C4), octyl (C8) and octadecyl (C18) silane groups are most commonly used.

TLS Online TPP Program

#Id: 2811

#Unit 13. Methods in Biology

In RPLC mobile phase is commonly water or aqueous buffers, methanol, acetonitrile or tetrahydrofuran, or mixtures of them.