Population doubling time in Geometric growth

d = 70/λ

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#Id: 2524

#Unit 13. Methods in Biology

Slab gels can develop a “Smiling Effect” When in a gel more Joule heat is produced than can be dissipated, leads to the higher mobility of the ions in the center, resulting in an uneven distribution of the zones, These zones bend up at the two lateral sides, causing “smiling.” 

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#Id: 2525

#Unit 13. Methods in Biology

The PCR consists of three defined sets of times and temperatures, termed steps

denaturatio

Annealing 

extension

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#Id: 2526

#Unit 13. Methods in Biology

The PCR involves two oligonucleotide primers, 17–30 nucleotides in length, which flank the DNA sequence that is to be amplified.

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#Id: 2527

#Unit 13. Methods in Biology

Duration of denaturation step in the first cycle of PCR is usually 2 min at 94 °C.

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#Id: 2528

#Unit 13. Methods in Biology

The duration of annealing step is usually 1 min during the first as well as the subsequent cycles of PCR. 

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#Id: 2529

#Unit 13. Methods in Biology

The duration of primer extension is usually 2 min at 72°C