Quaternary- Extinctions of large mammals, and birds; evolution of Homo sapiens, spread out of Africa

TLS Online TPP Program

#Id: 913

#Unit 13. Methods in Biology

bromodeoxyuridine (BrdU) is rapidly phosphorylated and incorporated in its place into newly synthesized DNA hence BrdU is used to detect replicating cells.

TLS Online TPP Program

#Id: 914

#Unit 13. Methods in Biology

BrdU can CAUSE light induced cell death. 

TLS Online TPP Program

#Id: 915

#Unit 13. Methods in Biology

If cells that have incorporated a high level of BrdU are exposed to light, they will photolyse; this has been used to selectively kill newly dividing cells.

TLS Online TPP Program

#Id: 916

#Unit 13. Methods in Biology

Propidium iodide is a fluorescent dye with a flat, planar structure that slides between the rungs of (or intercalates into) the DNA ladder in a quantitative manner frequently used in FACS. 

TLS Online TPP Program

#Id: 917

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.

TLS Online TPP Program

#Id: 918

#Unit 13. Methods in Biology

Propidium iodide-labeled cells in order as

1. Apoptotic cells

2. G1/G0

3. S

4. G2/M