Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

TLS Online TPP Program

#Id: 2830

#Unit 13. Methods in Biology

Affinity chromatography used for the purification of enzymes, nucleotides, nucleic acids, immunoglobulins, membrane receptors and even to whole cells and cell fragments.

TLS Online TPP Program

#Id: 2831

#Unit 13. Methods in Biology

Principle of purification of an enzyme by affinity chromatography.

TLS Online TPP Program

#Id: 2832

#Unit 13. Methods in Biology

Examples of group-specific ligands commonly used in affinity chromatography

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#Id: 2833

#Unit 13. Methods in Biology

The peptide or protein (known as a ‘ tag’) can be placed at the N- or C-terminal ends and provides a means for the fusion protein to be selectively purified from the cell extract by affinity chromatography.

TLS Online TPP Program

#Id: 2834

#Unit 13. Methods in Biology

Fusion Constructs to Aid Protein Purification can be created by using Glutathione- S -transferase (GST), Maltose-Binding Protein (MBP), Poly-Histidine Tag (His-tag), FLAG ®

TLS Online TPP Program

#Id: 2835

#Unit 13. Methods in Biology

GST tagged protein are separated by using Glutathione (a tripeptide with a g-peptide linkage, g -Glu-Cys-Gly), displacing the bound GST moiety.