Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

TLS Online TPP Program

#Id: 6014

#Unit 3. Fundamental Processes

Helicases are loaded during G1 are only activated to unwind DNA and initiate replication after cells pass from the G1 to the S phase of the cell cycle in effect with S phase CDKC

TLS Online TPP Program

#Id: 6010

#Unit 3. Fundamental Processes

In mammalian cells, Cdc6 is controlled differently; it is phosphorylated during S phase, and as a result it is degraded by the ubiquitination pathway.  Cdt1 is initially stabilized by the protein Geminin, which prevents its degradation, and subsequent Geminin binding prevents its reuse

TLS Online TPP Program

#Id: 902

#Unit 13. Methods in Biology

Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

TLS Online TPP Program

#Id: 10046

#Unit 4. Cell Communication and Cell Signaling

In the absence of Delta, 

TM subunit of Notch on the surface of a responding cell is non-covalently associated with its extracellular subunit; extracellular domain is folded

TLS Online TPP Program

#Id: 2551

#Unit 13. Methods in Biology

5′ nuclease assay (TaqMan assay). PCR is undertaken with a fluorescence reporter/quencher pair (called RQ probe).