TLS Online TPP Program

#Id: 4631

#Unit 13. Methods in Biology

Competitive ELISA. In this technique, antibody is first incubated in solution with a sample containing antigen. The antigen-antibody mixture is then added to an antigen coated microtiter well.
The more antigen present in the sample, the less free antibody will be available to bind to the antigen-coated well. 
Addition of an enzyme-conjugated secondary antibody (Ab2) specific for the isotype of the primary antibody can be used to determine the amount of primary antibody bound to the well as in an indirect ELISA. 
In the competitive assay, however, the higher the concentration of antigen in the original sample, the lower the absorbance.

TLS Online TPP Program

#Id: 4632

#Unit 13. Methods in Biology

 What are the principles in flow cytometry?

• Light scattered by a laser or arc lamp

• Specific fluorescence detection

• Hydrodynamically focused stream of particles

• Electrostatic particle separation for sorting

• Multivariate data analysis capability

TLS Online TPP Program

#Id: 4633

#Unit 13. Methods in Biology

3H thymidine uptake assays were the first to be used routinely to measure cell division in lymphocyte cultures

TLS Online TPP Program

#Id: 4634

#Unit 13. Methods in Biology

Colorimetric Assays for Cell Division Are Rapid and Eliminate the Use of Radioactive Isotopes

TLS Online TPP Program

#Id: 4635

#Unit 13. Methods in Biology

Colorimetric Assays for Cell Division Are Rapid and Eliminate the Use of Radioactive Isotopes

TLS Online TPP Program

#Id: 4636

#Unit 13. Methods in Biology

Bromodeoxyuridine-Based Assays for Cell Division Use Antibodies to Detect Newly Synthesized DNA