Leber hereditary optic neuropathy (LHON)- People who have this disorder experience rapid loss of vision in both eyes, which results from the death of cells in the optic nerve.

TLS Online TPP Program

#Id: 920

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

TLS Online TPP Program

#Id: 921

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

TLS Online TPP Program

#Id: 922

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.

TLS Online TPP Program

#Id: 923

#Unit 13. Methods in Biology

The 51Cr Release Assay is used to measure Cell death not apoptosis

TLS Online TPP Program

#Id: 924

#Unit 13. Methods in Biology

Normal cells treated with sodium chromate allowed to absorb and induced to death signals as T-CTLs Death of the target cells is indicated by the release of 51Cr into the supernatant

TLS Online TPP Program

#Id: 925

#Unit 13. Methods in Biology

FITC binds to Annexin V to phosphatidyl serine which are found on apoptotic cells, analyzed by FACS