Fitness is as the number of offspring an individual leaves in the next generation.

TLS Online TPP Program

#Id: 2961

#Unit 3. Fundamental Processes

The key event in controlling replication is the behavior of the ORC complex at the origin.

Cdc6 protein is an unstable protein that is synthesized only in G1.

Cdc6 binds to ORC and allows MCM proteins.

Cdc6 binds to ORC and allows MCM proteins to bind.

Cdt1 facilitates MCM loading on origins

TLS Online TPP Program

#Id: 2962

#Unit 3. Fundamental Processes

When replication is initiated, Cdc6, and Cdt1 are displaced. The degradation of Cdc6 prevents reinitiation.

TLS Online TPP Program

#Id: 2963

#Unit 3. Fundamental Processes

The striking feature is that ORC remains bound at the origin through the entire cell Cycle.

TLS Online TPP Program

#Id: 2964

#Unit 3. Fundamental Processes

The major role of ORC is to identify the origin to the Cdc6 and MCM proteins that control initiation and licensing.

TLS Online TPP Program

#Id: 2965

#Unit 3. Fundamental Processes

In yeast, Cdc6 is a highly unstable protein, with a half-life of <5 minutes. It is synthesized during G1 and typically binds to ORC between the exit from mitosis and late G1.

TLS Online TPP Program

#Id: 2966

#Unit 3. Fundamental Processes

pathway. Cdt1  is initially stabilized by the protein Geminin, which prevents its degradation, and subsequent Geminin binding prevents its reuse.