If the middle slice is too narrow, there is no discernible morphogen gradient within it, and regeneration is abnormal.

TLS Online TPP Program

#Id: 926

#Unit 13. Methods in Biology

The TUNEL Assay measures Apoptotically Generated DNA Fragmentation.

TLS Online TPP Program

#Id: 927

#Unit 13. Methods in Biology

TUNEL method uses TdT to add BrdU to fixed and permeabilized cells which can only incorporated into fragmented DNA of apoptotic cells.

TLS Online TPP Program

#Id: 928

#Unit 13. Methods in Biology

BrdU is incorporated into the newly synthesized DNA, and is then detected with fluorescently labeled anti-BrdU antibodies

TLS Online TPP Program

#Id: 929

#Unit 13. Methods in Biology

Caspases western blotting assay is also used to detect apoptosis

TLS Online TPP Program

#Id: 2456

#Unit 13. Methods in Biology

The term electrophoresis describes the migration of a charged particle in medium (buffer/liquid) under the influence of an electric field.

TLS Online TPP Program

#Id: 2457

#Unit 13. Methods in Biology

In electrophoresis because of the varying charges and masses, of different molecules and particles of a mixture will migrate at different velocities under uniform force and will thus be separated into single fractions.