A. G. Tiselius (1937) Performed Moving boundary electrophoresis  in a U-shaped tube using Schlieren optics on both end for Measurements of the electrophoretic mobilities.

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#Unit 13. Methods in Biology

DIC microscopy easily defines the outlines of large organelles, such as the nucleus and vacuole.

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#Unit 13. Methods in Biology

Formaldehyde a common fixative, crosslinks amino groups on adjacent molecules

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#Unit 13. Methods in Biology

Histological samples are often stained with hematoxylin and eosin (“H&E stain”).

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#Unit 13. Methods in Biology

Hematoxylin binds to basic amino acids (lysine and arginine).

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#Unit 13. Methods in Biology

Eosin binds to acidic molecules (DNA and side chains of aspartate and glutamate).

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#Unit 13. Methods in Biology

Darkfield microscopy is used for very thin spirochetes, such as Treponema pallidum, the causative agent of syphilis.