TEMED catalyzes the decomposition of the persulfate ion to give a free radical (i.e., a molecule with an unpaired electron):

TLS Online TPP Program

#Id: 2807

#Unit 13. Methods in Biology

The pH of the buffer selected as eluent should be at least one pH unit above or below the isoionic point of the analytes.

TLS Online TPP Program

#Id: 2808

#Unit 13. Methods in Biology

Reversed-phase liquid chromatography form of liquid chromatography, which has many similarities with hydrophobic interaction chromatography (HIC).

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#Id: 2809

#Unit 13. Methods in Biology

In RPLC the stationary phase is non-polar and the mobile phase relatively polar, hence the name reversed-phase

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#Id: 2810

#Unit 13. Methods in Biology

In RPLC stationary phase most commonly used is in the bonded-phase form, in which alkylsilane groups are chemically attached to silica. Butyl (C4), octyl (C8) and octadecyl (C18) silane groups are most commonly used.

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#Id: 2811

#Unit 13. Methods in Biology

In RPLC mobile phase is commonly water or aqueous buffers, methanol, acetonitrile or tetrahydrofuran, or mixtures of them.

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#Id: 2812

#Unit 13. Methods in Biology

 In RPLC the organic solvent is referred to as an organic modifier.