The higher no. of band will be observed in gradient gel electrophoresis comparison to uniform gel SDS and Native gel electrophoresis.

TLS Online TPP Program

#Id: 555

#Unit 2. Cellular Organization

The calcium-dipicolinic acid complex play a role in the heat resistance of endospore.

TLS Online TPP Program

#Id: 556

#Unit 2. Cellular Organization

lacZ = b-galactosidase

lacY = galactoside permease

lacA = galactoside transacetylase

All 3 genes are transcribed together producing 1 mRNA, a polycistronic message 

TLS Online TPP Program

#Id: 557

#Unit 2. Cellular Organization

The lacA gene encodes thiogalactoside transacetylase, which rids the cell of toxic thiogalactosides that also get transported by lacY.

TLS Online TPP Program

#Id: 558

#Unit 2. Cellular Organization

There are 4 genes (I, Z, Y, A), 2 Promoters and 3 lac operators in Lac operon

The major O1 lac operator lies adjacent to promoter

Two auxiliary O2 and O3 lac operators 

All three operators are required for optimum repression 

TLS Online TPP Program

#Id: 559

#Unit 2. Cellular Organization

The O1 operator extends from position -5 just upstream of the mRNA start point to position +21 within the transcription unit.

TLS Online TPP Program

#Id: 560

#Unit 2. Cellular Organization

When polymerase binding causes transition to the open complex and initiates transcription. This gives a low level of constitutive expression called the basal level. 

Binding of RNA polymerase is the rate-limiting step in this case.