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IEF is a highly sensitive analytical technique and is particularly useful for studying  microheterogeneity in a protein. For example, a protein may show a single band on an SDS gel but may show three bands on an IEF gel. This may occur, for example, when a protein exists in mono-, di- and tri-phosphorylated forms. 


#Unit 13. Methods in Biology #Electrophoresis #Part B Pointers
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#Unit 7. System Physiology – Animal

The concentrating mechanism depends upon the maintenance of a gradient of increasing osmolality along the medullary pyramids










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#Id: 1585

#Unit 5. Developmental Biology

Ability to initiate a primitive streak is found throughout the marginal zone.


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#Id: 4021

#Unit 2. Cellular Organization

Active genes are often found at the borders of chromosome territories, and active genes from several chromosomes may cluster in inter-chromosomal territories that are enriched in transcription machinery.

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#Id: 5979

#Unit 10. Ecological Principles

Decomposition Decomposition is the physical and chemical breakdown of dead plant and animal biomass. Decomposition is increased by High Temperatures, Soil Moisture, and Soil Nutrients.




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#Id: 9049

#Unit 4. Cell Communication and Cell Signaling

Three principal classes of G PCR that bind their ligand in different ways:

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