The specificity of the PCR depends crucially upon the primers. The following factors are important in choosing effective primers

Primers should be 17 to 30 nucleotides in length

A GC content of about 50% is ideal. For primers with a low GC content, it is desirable to choose a long primer to avoid a low melting

 temperature.

Sequences with long runs (i.e., more than three or four) of a single nucleotide should be avoided.

TLS Online TPP Program

#Id: 10054

#Unit 4. Cell Communication and Cell Signaling

Cleavage by the γ-secretase complex releases the Notch cytosolic segment, which translocates to the nucleus, interacts with a single DNA-binding transcription factor, CSL.

TLS Online TPP Program

#Id: 2959

#Unit 3. Fundamental Processes

A nucleus injected into a Xenopus egg can replicate only once unless the nuclear membrane is permeabilized to allow subsequent replication cycles

TLS Online TPP Program

#Id: 3593

#Unit 3. Fundamental Processes

The side chains of amino acids have little or no specific affinity for the purine and pyrimidine bases found in RNA.

TLS Online TPP Program

#Id: 10115

#Unit 6. System Physiology – Plant

Rapidly dividing tissues, shoot apical meristems, young leaves, and developing fruits and seeds are the primary sites of IAA synthesis.

TLS Online TPP Program

#Id: 8883

#Unit 2. Cellular Organization

Once mitotic CDKs are activated at the end of G2 , they phosphorylate specific serine residues in all 3 nuclear lamins.