The specificity of the PCR depends crucially upon the primers. The following factors are important in choosing effective primers

Primers should be 17 to 30 nucleotides in length

A GC content of about 50% is ideal. For primers with a low GC content, it is desirable to choose a long primer to avoid a low melting

 temperature.

Sequences with long runs (i.e., more than three or four) of a single nucleotide should be avoided.

TLS Online TPP Program

#Id: 8421

#Unit 10. Ecological Principles

Human populations can exist at equilibrium densities in one of two ways:

1. High birth and high death rates

2. Low birth and low death rate

TLS Online TPP Program

#Id: 8422

#Unit 10. Ecological Principles

The difference between the birth rate and the death rate determines the rate of natural increase or decrease

TLS Online TPP Program

#Id: 8423

#Unit 10. Ecological Principles

Parental investment - The amount of time and energy given to an offspring by its parents. 

Longevity - The life span of an organism. Also known as Life expectancy

TLS Online TPP Program

#Id: 8424

#Unit 10. Ecological Principles

TLS Online TPP Program

#Id: 8425

#Unit 10. Ecological Principles

Fecundity - The number of offspring produced by an organism per reproductive episode. 

Parity - The number of reproductive episodes an organism experiences. 

TLS Online TPP Program

#Id: 8426

#Unit 10. Ecological Principles

Senescence - A gradual decrease in fecundity and an increase in the probability of mortality.