Extension step in the second cycle will produce a product that will be much shorter than the 'long product'; this is the 'correct' PCR product and represents the target sequence

TLS Online TPP Program

#Id: 2926

#Unit 3. Fundamental Processes

As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases. 

TLS Online TPP Program

#Id: 2930

#Unit 3. Fundamental Processes

The dat locus contains DnaA-binding sites that titrate availability of DnaA protein.

TLS Online TPP Program

#Id: 2931

#Unit 3. Fundamental Processes

Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.

TLS Online TPP Program

#Id: 2932

#Unit 3. Fundamental Processes

Replication in bacteria and in eukaryotes is licensed and permitted to occur only once per cell cycle.

TLS Online TPP Program

#Id: 2933

#Unit 3. Fundamental Processes

The E. coli oriC is fully methylated at the beginning of replication.

TLS Online TPP Program

#Id: 2934

#Unit 3. Fundamental Processes

oriC is hemimethylated and remains in that condition for approximately 13 minutes.