7-dcaza guanosine triphosphate (7-deaza OTP) can be used in place of OTP; it reduces hairpin loop formation without affecting the Watson-Crick base pairing required for annealing and primer extension. 

TLS Online TPP Program

#Id: 8888

#Unit 2. Cellular Organization

A key function of mitotic CDKs, together with other protein kinases and ubiquitin-protein ligases, is to induce the formation of the mitotic spindle, also known as the mitotic apparatus.

TLS Online TPP Program

#Id: 8889

#Unit 2. Cellular Organization

Centrosomes contain γ tubulin, which forms specific ringlike complexes (γ TuRCs) that, have the ability to nucleate microtubules and form a bipolar spindle

TLS Online TPP Program

#Id: 8890

#Unit 2. Cellular Organization

Mitotic spindle attach to chromosomes via specialized protein structures known as kinetochores

TLS Online TPP Program

#Id: 8891

#Unit 2. Cellular Organization

Kinetochores assemble on regions of chromosomal DNA marked by a centromere-specific H3 histone variant called CENP-A.

TLS Online TPP Program

#Id: 8892

#Unit 2. Cellular Organization

Amphitelic attachment, 

sister kinetochores attach to microtubules emanating from opposite spindle poles, they are stably attached

TLS Online TPP Program

#Id: 8893

#Unit 2. Cellular Organization

Merotelic attachment 

A kinetochore can attach to microtubules emanating from both poles at the same time