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5′ nuclease assay (TaqMan assay). PCR is undertaken with a fluorescence reporter/quencher pair (called RQ probe). 




#Unit 13. Methods in Biology #PCR #Part B Pointers
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#Unit 7. System Physiology – Animal

Tyrosine is transported into catecholamine-secreting neurons via a Na+ - dependent carrier



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#Unit 7. System Physiology – Animal

Rate-limiting step in synthesis of catecholamines:  Tyrosine is converted to dihydroxy-phenylalanine (DOPA) by tyrosine hydroxylase

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#Unit 7. System Physiology – Animal

DOPA is converted to dopamine in the cytoplasm of the cells by DOPA decarboxylase (amino acid decarboxylase)



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#Unit 7. System Physiology – Animal

DOPA is converted to dopamine in the cytoplasm of the cells by DOPA decarboxylase (amino acid decarboxylase)

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#Unit 7. System Physiology – Animal

Dopamine is converted to norepinephrine by dopamine β -hydroxylase





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#Unit 7. System Physiology – Animal

Norepinephrine is the only small-molecule transmitter that is synthesized in synaptic vesicles


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