Molecular beacons consist of a stem (5–7 bp), and a loop (18–30 base pairs) and are labeled at the 5′ and 3′ ends by fluorophores and quenchers, respectively. 

TLS Online TPP Program

#Id: 5537

#Unit 7. System Physiology – Animal

Thick segment of the ascending limb of loop of Henle, has thick epithelial cells that have high metabolic activity and are capable of active reabsorption of sodium, chloride, and potassium

TLS Online TPP Program

#Id: 5538

#Unit 7. System Physiology – Animal

In the thick segment of the ascending loop of Henle  reabsorption mechanism is load-dependent.

TLS Online TPP Program

#Id: 5539

#Unit 7. System Physiology – Animal

In the thick ascending loop,  Luminal membrane contains a three-ion cotransporter  known as Na+-K+-2Cl- cotransporter

TLS Online TPP Program

#Id: 5540

#Unit 7. System Physiology – Animal

Thick ascending limb is called the diluting segment because NaCl is reabsorbed but water is not reabsorbed along with it 

TLS Online TPP Program

#Id: 5541

#Unit 7. System Physiology – Animal

The Cl – moves into the interstitium via ClC-Kb channels & Barttin, a protein in the cell membrane, is essential for normal ClC-Kb

function

TLS Online TPP Program

#Id: 5542

#Unit 7. System Physiology – Animal

The thick ascending limb of the loop of Henle is the site of action of the powerful “loop” diuretics such as

Furosemide

Ethacrynic acid

Bumetanide