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TLS Online TPP Program

#Id: 2565

Molecular beacons consist of a stem (5–7 bp), and a loop (18–30 base pairs) and are labeled at the 5′ and 3′ ends by fluorophores and quenchers, respectively. 

#Unit 13. Methods in Biology #Fluorescence #Part B Pointers
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TLS Online TPP Program

#Id: 8323

#Unit 2. Cellular Organization
TLS Online TPP Program

#Id: 8324

#Unit 2. Cellular Organization

The rate of addition of monomers is given by
rate constant kon (M–1 sec–1 )
The rate of loss is given by koff (sec–1)
TLS Online TPP Program

#Id: 8325

#Unit 2. Cellular Organization

Critical concentration (Cc)
minimal concentration of monomers at which filaments form
TLS Online TPP Program

#Id: 8326

#Unit 2. Cellular Organization

The two ends of microfilament polymerize at different rates:
fast-growing end - plus end
slow-growing end - minus end
TLS Online TPP Program

#Id: 8327

#Unit 2. Cellular Organization
TLS Online TPP Program

#Id: 8328

#Unit 2. Cellular Organization

Hydrolysis of the bound nucleotide reduces the binding affinity of the subunit for neighbouring subunits and makes it more likely to dissociate from each end of the filament.
T form that adds to the filament and the D form that leaves.