The logic for hotstart PCR is as follows, most DNA samples used as template will have some single-stranded DNA. 

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#Unit 8. Inheritance Biology

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#Unit 8. Inheritance Biology

Prototrophs– Wild type

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#Unit 8. Inheritance Biology

Auxotrophs- Nutritional mutant that disrupts the ability to synthesize an essential biological molecule = mutant type

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#Unit 8. Inheritance Biology

Auxotrophs or Mutant bacterial strains can be isolated on the basis of their nutritional requirements.

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#Unit 8. Inheritance Biology

Plasmid Small, circular DNA molecule found in bacterial cells that is capable of replicating independently from the bacterial chromosome.

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#Unit 8. Inheritance Biology

Episome Plasmid capable of integrating into a bacterial chromosome.