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#Id: 2595

Oligonucleotide-directed mutagenesis
The basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .

#Unit 13. Methods in Biology #PCR #Part B Pointers

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#Unit 8. Inheritance Biology

Segmental duplications have played an important role in the evolution of human chromosomes; about 4% of the human genome consists of segmental duplications.

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#Id: 6917

#Unit 8. Inheritance Biology

Unbalanced gene dosage leads to developmental abnormalities.

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#Unit 8. Inheritance Biology

Chromosome deletion

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#Id: 6919

#Unit 8. Inheritance Biology

Chromosome deletion - Loss of a chromosome segment.

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#Id: 6920

#Unit 8. Inheritance Biology

Deletion include,
Terminal deletion

ntercalary/interstitial deletion

Microdeletion

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#Id: 6921

#Unit 8. Inheritance Biology

Cri-du- chat syndrome- Deletion on 5, short arm

Wolf– Hirschhorn syndrome- Deletion on 4, short arm

Williams– Beuren syndrome - Deletion on 7, long arm

Prader– Willi syndrome- Deletion on 15 long arm

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Oligonucleotide-directed mutagenesis
The basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .

Pointers

Oligonucleotide-directed mutagenesisThe basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .

Oligonucleotide-directed mutagenesis
The basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .