Pointers

TLS Online TPP Program

#Id: 2595

Oligonucleotide-directed mutagenesis
The basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .

#Unit 13. Methods in Biology #PCR #Part B Pointers

PYQ Links

PYQ 1

Extensive Links

Link 1

More Pointers

TLS Online TPP Program

#Id: 428

#Unit 2. Cellular Organization

Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA between nucleosomes, a region known as linker DNA.

TLS Online TPP Program

#Id: 429

#Unit 2. Cellular Organization

Mononucleosomes typically have 200 bp DNA. End-trimming reduces the length of DNA first to 165 bp and then generates core particles with 146 bp.

TLS Online TPP Program

#Id: 430

#Unit 2. Cellular Organization

Mnase digestion result on centrifugation and electrophoresis

TLS Online TPP Program

#Id: 431

#Unit 2. Cellular Organization

Core DNA has an invariant length of 146 bp the minimum length of DNA needed to form a stable monomeric nucleosome, and is relatively resistant to digestion by nucleases.

TLS Online TPP Program

#Id: 432

#Unit 2. Cellular Organization

Core DNA invariant length of 146 bp contains core and linker histones so Mnase cuts after H1 binding site.

TLS Online TPP Program

#Id: 433

#Unit 2. Cellular Organization

The nucleosome is a disc with DNA organized into 12/3 turns around the surface.

Triyambak Life Sciences
Nurturing Life Sciences

TLS Online
Nurturing Life Sciences

Oligonucleotide-directed mutagenesis
The basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .

Pointers

Oligonucleotide-directed mutagenesisThe basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .

Oligonucleotide-directed mutagenesis
The basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .