Oligonucleotide-directed mutagenesis.

This technique requires a knowledge of nucleotide sequence, since an oligonucleotide may then be synthesized with the base mutation. 

Annealing of the oligonucleotide to complementary (except for the mutation) single-stranded DNA provides a primer for DNA polymerase to produce a new strand and thus incorporates the primer with the mutation.

TLS Online TPP Program

#Id: 9252

#Unit 6. System Physiology – Plant

In Crassulacean acid metabolism (CAM), CO2 uptake is separated temporally and its solely depends on PEPCase circadian modification

TLS Online TPP Program

#Id: 9253

#Unit 6. System Physiology – Plant

TLS Online TPP Program

#Id: 9254

#Unit 6. System Physiology – Plant

TLS Online TPP Program

#Id: 9255

#Unit 6. System Physiology – Plant

In CAM plants, Nocturnal phosphorylation of PEPCase controls primary carboxylation. PEPCase‐kinase transcripts accumulate during the night, controlled by the circadian oscillator and metabolites.

TLS Online TPP Program

#Id: 9256

#Unit 6. System Physiology – Plant

The concentration at which net change in CO2 is 0 defines the CO2 compensation point and reflects the CO2 concentration at which the rate of gross photosynthetic CO2  uptake exactly equals the respiration rate.

TLS Online TPP Program

#Id: 9257

#Unit 6. System Physiology – Plant

Diurnal changes in xanthophyll content as a

function of PPFD