Oligonucleotide-directed mutagenesis.

This technique requires a knowledge of nucleotide sequence, since an oligonucleotide may then be synthesized with the base mutation. 

Annealing of the oligonucleotide to complementary (except for the mutation) single-stranded DNA provides a primer for DNA polymerase to produce a new strand and thus incorporates the primer with the mutation.

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#Unit 11. Evolution and Behavior

These pieces— the exons —are separated by stretches of noncoding DNA— the introns

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#Unit 6. System Physiology – Plant

NodA is an N-acyltransferase that catalyzes the addition of a fatty acyl chain.

NodB is a chitin-oligosaccharide deacetylase that removes the acetyl group from the terminal nonreducing sugar.

NodC is a chitin-oligosaccharide synthase that links N-acetyl-D-glucosamine monomers.

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#Unit 2. Cellular Organization

RFLPs and SNPs can be the basis for linkage maps and are useful for establishing parent–offspring relationships.

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#Unit 5. Developmental Biology

Competence 

The ability to respond to a specific inductive signal

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#Unit 2. Cellular Organization

Phosphorylation of Whi5 promotes its export out of the nucleus, allowing the transcription factor complex SBF to induce transcription of the G1/S phase cyclin genes CLN1 and CLN2.