RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. 

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#Unit 3. Fundamental Processes

The 3’–5’ exonucleolytic proofreading activity is found in E, coded by the dnaQ gene

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#Id: 704

#Unit 3. Fundamental Processes

Mutations in dnaQ showed increased mutation frequency with by103-folds

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#Unit 3. Fundamental Processes

Proofreading typically decreases the error rate in replication from10-5 to 10-7 per base pair replicated.

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#Id: 706

#Unit 3. Fundamental Processes

Repair Systems such as MMR reduces error rate of replication to 10-9 per base pair replicated

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#Id: 707

#Unit 3. Fundamental Processes

SeqA binds to hemimethylated DNA and is required for delaying rereplication.

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#Id: 708

#Unit 3. Fundamental Processes

seqA is part of a negative regulatory circuit that prevents origins from being remethylated.