RACE can be used to amplify unknown 5' (5'-RACE) or 3' (3'-RACE) parts of RNA molecules where part of the RNA sequence is known and targeted by a GSP gene-specific primer.

TLS Online TPP Program

#Id: 6475

#Unit 3. Fundamental Processes

RNA Polymerase III promoters are bipartite

The exception is in yeast, where the TATA box is more typically found at -90.

TLS Online TPP Program

#Id: 6476

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6477

#Unit 3. Fundamental Processes

Start point, is the first base of mRNA to be A Initiator (Inr) is Py2CAPy5, where Py stands for any pyrimidine. The Inr is contained between positions -3 and +5. TATA box, usually located, 25 bp upstream in its location at -25

TLS Online TPP Program

#Id: 6478

#Unit 3. Fundamental Processes

Promoters that do not contain a TATA element are called TATA-less promoters. Surveys of promoter sequences suggest that 50% or more of promoters may be TATA-less.

TLS Online TPP Program

#Id: 6479

#Unit 3. Fundamental Processes

Constitutive active housekeeping genes and developmentally regulated genes have TATA-less promoters 

TLS Online TPP Program

#Id: 6480

#Unit 3. Fundamental Processes