To more accurately validate the predicted target genes of miRNAs and exactly determine the cleavage sites within the targets, an integrated strategy comprising RLM-RACE, Poly(A) Polymerase-Mediated (PPM)-RACE, and qRT-PCR was developed.

TLS Online TPP Program

#Id: 10249

#Unit 6. System Physiology – Plant

Two regulatory pathways exist for ABA catabolism:

the oxidative pathway and the sugar conjugation pathway

TLS Online TPP Program

#Id: 10250

#Unit 6. System Physiology – Plant

vp12 mutant have low chlorophyll content and reduced capacity for synthesis of carotenoids and ABA

TLS Online TPP Program

#Id: 10251

#Unit 6. System Physiology – Plant

The pyrabactin resistance (PYR)/PYR‐related (PYL)/regulatory

component of ABA receptor (RCAR) family of ABA receptors (PYR/PYL/RCAR protein family) have ABA‐binding activity

TLS Online TPP Program

#Id: 10252

#Unit 6. System Physiology – Plant

A signaling complex of PYR/PYL/RCAR, PP2C, and SnRK2 is important for ABA perception and the initial signal transduction process.

TLS Online TPP Program

#Id: 10253

#Unit 6. System Physiology – Plant

PYR/PYL/RCARs function as cytoplasmic ABA receptors and inhibit PP2C‐mediated inactivation of SnRK2 in an ABA‐dependent manner

TLS Online TPP Program

#Id: 10254

#Unit 6. System Physiology – Plant

SnRK2s are activated by dehydration, salinity, and ABA