To more accurately validate the predicted target genes of miRNAs and exactly determine the cleavage sites within the targets, an integrated strategy comprising RLM-RACE, Poly(A) Polymerase-Mediated (PPM)-RACE, and qRT-PCR was developed.

TLS Online TPP Program

#Id: 7490

#Unit 4. Cell Communication and Cell Signaling

P nucleotide addition results when the DNA hairpin at the coding joint is cleaved asymmetrically.

TLS Online TPP Program

#Id: 7491

#Unit 4. Cell Communication and Cell Signaling

Non - templated N nucleotide addition in heavy chains results from TdT activity. 

TLS Online TPP Program

#Id: 7492

#Unit 4. Cell Communication and Cell Signaling

Exonuclease trimming sometimes occurs at the VDJ and VJ junctions, causing loss of nucleotides.

TLS Online TPP Program

#Id: 7493

#Unit 4. Cell Communication and Cell Signaling

Combinatorial diversity: The same heavy chain can combine with different light chain

TLS Online TPP Program

#Id: 7494

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 7495

#Unit 4. Cell Communication and Cell Signaling

This recombinational event is catalyzed by a set of enzymes, many of which are also involved in nonhomologous end joining (NHEJ) DNA repair functions that occur in all cells.