TLS Online TPP Program

#Id: 2615


In plants, the perfect complementarity between most miRNAs and their targets enables the accurate predictions of their targets, while slicing of the targeted mRNAs facilitates target validation through RNA Ligase-Mediated (RLM)-Rapid Amplification of cDNA Ends (RACE) method.

#Unit 13. Methods in Biology #PCR #Part B Pointers
More Pointers
TLS Online TPP Program

#Id: 7710

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 7711

#Unit 1. Molecules and their Interaction Relevant to Biology

                                                                 α-Turn

Contains hydrophilic amino acids and protrudes outside the protein surface like a hook.

Consists of 5 amino acids, generally termed as i, i+1, i+2, i+3 and i+4

H-bond is seen between i and i+4 (First and Fifth residue)

The conformation of i+1, i+2 and i+3 are crucial and on the basis of this, they are classified into

9 groups

TLS Online TPP Program

#Id: 7712

#Unit 1. Molecules and their Interaction Relevant to Biology

                                                γ-Turn


Consists of 3 amino acids, generally termed as i, i + 1 and i + 2

H-bond is seen between i and i + 2 (First and Third residue)

The conformation of i + 1 is crucial and on the basis of this, they are classified into 2 groups

The dihedral angles of residue i + 1 are (70, -60) and (-70, 60) of the classical and inverse gamma turns

TLS Online TPP Program

#Id: 7713

#Unit 1. Molecules and their Interaction Relevant to Biology

                                                        𝝅-Turn

Consists of 6 amino acids, generally termed as i, i + 1 , i + 2 i + 3 i + 4 and i + 5

H-bond is seen between i and i + 5 (First and sixth residue)

TLS Online TPP Program

#Id: 8443

#Unit 1. Molecules and their Interaction Relevant to Biology

The 310 helix has 3 residues per tum and contains 10 atoms between the hydrogen bond donor and acceptor, hence its name.

TLS Online TPP Program

#Id: 8444

#Unit 1. Molecules and their Interaction Relevant to Biology

Both the pi helix and the 310 helix occur rarely and usually only at the ends of a helices or as single-tum helices. They are not energetically favorable, since the backbone atoms are too tightly packed in the 310 helix and so loosely packed in the n helix that there is a hole through the middle. 
Only in the a helix are the backbone atoms properly packed to provide a stable structure.