After you have identified the physiological transcription start site by S1 mapping or primer extension you can use run-off transcription in vitro.

TLS Online TPP Program

#Id: 5913

#Unit 3. Fundamental Processes

DnaG primase associates transiently with the replication complex, and typically synthesizes a 10-base primer. Primers start with the sequence pppAG positioned opposite the sequence 3’–GTC-5’ in the template.

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#Id: 10268

#Unit 6. System Physiology – Plant

ABA also regulate the NHX1 Na+/H+ antiporter and

other ion transporters during salt stress

TLS Online TPP Program

#Id: 4711

#Unit 13. Methods in Biology

Common colorimetric and UV absorption assays

TLS Online TPP Program

#Id: 7698

#Unit 1. Molecules and their Interaction Relevant to Biology

                                                     Greek Key Motif

                                                        

TLS Online TPP Program

#Id: 5679

#Unit 7. System Physiology – Animal

Cells in the stria vascularis have a high concentration of Na+-K+ ATPase