Bacteriostatic Drugs

Drugs that inhibit the growth of the pathogen without causing cell death are called bacteriostatic. These drugs target metabolic pathways that are necessary for bacterial growth but not for bacterial survival.

TLS Online TPP Program

#Id: 867

#Unit 13. Methods in Biology

Size of the primer negatively affects the efficiency of PCR.

TLS Online TPP Program

#Id: 868

#Unit 13. Methods in Biology

PCR amplification is mathematically counted as 2n So 10 cycle leads to 1024 times amplification

TLS Online TPP Program

#Id: 869

#Unit 13. Methods in Biology

The efficiency of PCR can be calculated by using Y = X (1+ E)n X is number of templates, E is mean PCR efficiency, n is number is PCR cycle. 

 

TLS Online TPP Program

#Id: 870

#Unit 13. Methods in Biology

Variants of PCR has been devised for 

a. Efficiency

b. Sequence specific amplification

c. Mutation analysis

TLS Online TPP Program

#Id: 871

#Unit 13. Methods in Biology

Variants of PCR has been devised to enhance efficiency are 

a. qPCR with SYBR, Taqman and MB 

b. Nested PCR

c. Touchdown PCR

d. Hot-start PCR 

TLS Online TPP Program

#Id: 872

#Unit 13. Methods in Biology

Variants of PCR has been devised for sequence specific amplification are Inverse PCR, Anchored PCR, RT-PCR RACE (3’ and 5’) RLM-RACE, Multiplex PCR, Colony PCR.