Selectable reporter genes

Selectable marker genes include those conferring resistance to methotrexate (dhfr, isolated from Tn7 or plasmid R67); the antibiotics kanamycin (nptII, encoding neomycin phosphotransfcrase) and hygromycin (hptIV, encoding hygromycin phosphotransferase, .isolated from E. coli);

TLS Online TPP Program

#Id: 9811

#Unit 6. System Physiology – Plant

A toggle model is for phytochrome interconversion

The structure of the b-hairpin region of Pr is changed to an a helix during the conversion of Pr to Pfr, which initiates other conformational changes in the protein.

TLS Online TPP Program

#Id: 9812

#Unit 6. System Physiology – Plant

There are five phytochrome isoforms in angiosperms

TLS Online TPP Program

#Id: 9813

#Unit 6. System Physiology – Plant

PHYA is the only gene that encodes a Type I phytochrome and predominantly perceives far-red light.

Phytochrome B predominantly perceives red light

TLS Online TPP Program

#Id: 9814

#Unit 6. System Physiology – Plant

The PHYA gene is transcriptionally active in dark-grown seedlings, but its expression is strongly inhibited in the light in monocots.

TLS Online TPP Program

#Id: 9815

#Unit 6. System Physiology – Plant

The PRD domain of phyB serve as an NLS. 

Conversion of Pr to Pfr by red light expose the functional NLS of the PRD domain of phyB, facilitating phyB’s import into the nucleus.

TLS Online TPP Program

#Id: 9816

#Unit 6. System Physiology – Plant

PRD domain of phyA cannot act as an NLS, so to transport it into the nucleus requires FARRED ELONGATED HYPOCOTYL1 (FHY1) and its homolog FHY1-LIKE (FHL),