Nurturing Life Sciences
#Id: 2736
The run-on reaction is usually done in the presence of labeled nucleotides so the products will be labeled.
#Id: 2629
#Unit 13. Methods in Biology
End-labelling of a gene probe at the 5′ end with alkaline phosphatase and polynucleotide kinase.
#Id: 2630
End-labelling of a gene probe at the 3′ end using terminal transferase. Note that the addition of a labeled dNTP at the 3′ end alters the sequence of the gene probe.
#Id: 2631
S1 mapping is used to locate the 5’- or 3’-ends of RNAs and to quantify the amount of a given RNA in cells at a given time.
#Id: 2632
The principle behind the S1 mapping method is to label a single stranded DNA probe that can hybridize only to the transcript of interest.
#Id: 2633
The probe must span the sequence where the transcript starts or ends. After hybridizing the probe to the transcript, one applies S1 nuclease, which degrades only single-stranded DNA and RNA.
#Id: 2634
