Methylation interference assay Dimethyl sulphate (DMS) methylates purines on DNA strand. Methylate DNA prior to adding protein. Protein binding is prevented at methylated sites. After treatment and removal of protein of interest, treat with piperidine or NaOH which cuts strand at modified bases.

TLS Online TPP Program

#Id: 10098

#Unit 4. Cell Communication and Cell Signaling

Adherens junctions 

Adhesion type- Cell-cell

Principal CAMs- Cadherins

Cytoskeletal attachment- Actin filament

TLS Online TPP Program

#Id: 10099

#Unit 4. Cell Communication and Cell Signaling

Adherens junctions

functions as a tension cable that can internally brace the cell and thereby control its shape

TLS Online TPP Program

#Id: 10100

#Unit 4. Cell Communication and Cell Signaling

Desmosomes

Adhesion type- Cell-cell

Cytoskeletal attachment- Intermediate filaments

TLS Online TPP Program

#Id: 10101

#Unit 4. Cell Communication and Cell Signaling

The key CAMs in desmosomes are the desmosomal cadherins, desmoglein and desmocollin

TLS Online TPP Program

#Id: 10102

#Unit 4. Cell Communication and Cell Signaling

Desmosomes

Function- Strength, durability, signaling

TLS Online TPP Program

#Id: 10103

#Unit 4. Cell Communication and Cell Signaling

Hemidesmosomes

Adhesion type- Cell-matrix

Cytoskeletal attachment- Intermediate filaments