UV radiation is used to cause DNA breakage. UV reacts quickly hence helps to catch momentary protein binding and can be used in vivo as it penetrates cell membranes easily. But gels from such assays are difficult to interpret.

TLS Online TPP Program

#Id: 9054

#Unit 4. Cell Communication and Cell Signaling

The activated β2-adrenergic receptor induces GDP-->GTP exchange on a stimulatory G protein (Gs).

TLS Online TPP Program

#Id: 9055

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 9056

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 9057

#Unit 4. Cell Communication and Cell Signaling

glutamate (E200) and arginine (R209) participate in binding of cAMP, which causes a conformational change in the loop that prevents binding of the loop to the C subunit.

TLS Online TPP Program

#Id: 9058

#Unit 4. Cell Communication and Cell Signaling

Epinephrine --> Gαs-GTP-->Adenylyl cyclase-->cAMP-->PKA

TLS Online TPP Program

#Id: 9059

#Unit 4. Cell Communication and Cell Signaling

PKA always phosphorylates a serine or threonine residue that is located within the common sequence motif: X-Arg-(Arg/Lys)-X-(Ser/Thr)-Φ