Ion exchangers often use Gradient elution than isocratic elution, while Gradient elution is based on gradient of pH and ionic strength.

TLS Online TPP Program

#Id: 2971

#Unit 3. Fundamental Processes

When initiation occurs, Cdc6 and Cdtl are displaced, returning the origin to the state of the postreplication complex, which contains only ORC. 

TLS Online TPP Program

#Id: 2972

#Unit 3. Fundamental Processes

In yeast, Cdc6 is rapidly degraded during S phase, and as a result it is not available to support reloading of MCM proteins. Thus, the origin crumot be used for a second cycle of initiation during S phase.

TLS Online TPP Program

#Id: 2973

#Unit 3. Fundamental Processes

in mammalian cells, Cdt1 is targeted for degradation by the action of a protein complex that is recruited to the origin of replication by PCNA, the eukaryotic counterpart of the bacterial beta-clamp.

TLS Online TPP Program

#Id: 2974

#Unit 3. Fundamental Processes

In Xenopus extracts, replication can be initiated if ORC is removed after it has loaded Cdc6 and MCM proteins.

TLS Online TPP Program

#Id: 2975

#Unit 3. Fundamental Processes

The MCM proteins, once activated, are required for elongation as well as for initiation.

TLS Online TPP Program

#Id: 2976

#Unit 3. Fundamental Processes

The MCM proteins continue to function at the two bidirectional replication forks as the replication helicase.