Principle of purification of an enzyme by affinity chromatography.

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#Id: 2740

#Unit 13. Methods in Biology

You can use S1 mapping or primer extension analysis to do this measurement, but you can also substitute a reporter gene for the natural gene, and then assay the activity of the reporter gene product.

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#Id: 2741

#Unit 13. Methods in Biology

Gene expression can be quantified by measuring the accumulation of the protein products of genes. Immunoblotting and immunoprecipitation 

are the favorite ways of accomplishing this task.

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#Id: 2742

#Unit 13. Methods in Biology

Random amplified polymorphic DNA (RAPD) is a DNA marker that is defined by differences between individuals in terms of DNA regions either being or not being; amplified in a polymerase chain reaction primed by random oligonucleotide (usually, 10-mer) sequences. 

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#Id: 2743

#Unit 13. Methods in Biology

Polymorphism is produced due to the complementary sequence for the primer used being

present in one strain (strain A) but not in the other (strain B). As a result, an amplification product will be detectable as a band in strain A, while strain B will not show the product.

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#Id: 2744

#Unit 13. Methods in Biology

RAPDs behave as dominant markers and are recognized by the primer used for amplification.

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#Id: 2745

#Unit 13. Methods in Biology

RAPD bands are treated as independent loci;